Inhibition of translation

Good L., Nielsen P.E. Inhibition of translation and bacterial growth by peptide nucleic acid targeted to ribosomal RNA. Proc. Natl Acad. Sci. USA 1998 95 2073-2076. 

What could be the signal for the induction of the cold shock proteins It has been observed that shifting E. coli cells from 37 to 5 °C results in an accumulation of 70S monosomes with a concomitant decrease in the number of polysomes [129]. Further, it has been shown that a cold shock response is induced when ribosomal function is inhibited, e.g. by cold-sensitive ribosomal mutations [121] or by certain antibiotics such as chloramphenicol [94]. These data indicate that the physiological signal for the induction of the cold shock response is inhibition of translation caused by the abrupt shift to lower temperature. Then, the cold shock proteins RbfA, CsdA and IF2 associate with the 70S ribosomes to convert the cold-sensitive nontranslatable ribosomes into cold-resistant translatable ribosomes. This in turn results in an increase in cellular protein synthesis and growth of the cells. 

Humphreys, D. T., Westman, B., Martin, D. I., and Preiss, T. (2008). Inhibition of translation initiation by a microRNA. In MicroRNAs From Basic Science to Disease Biology (K. Appasani, ed.), pp. 83—99. Cambridge University Press, Cambridge UK. 

Aktas, H., Fluckiger, R., Acosta, J. A., Savage, J. M., Palakurthi, S. S., and Halperin, J. A. (1998). Depletion of intracellular Ca2+ stores, phosphorylation of eIF2alpha, and sustained inhibition of translation initiation mediate the anticancer effects of clotrimazole. Proc. Natl Acad. Sci. USA 95, 8280-8285. 

Pniai RS, Bhattacharyya SN, Artus CG et al (2005) Inhibition of translational initiation by Let-7 MicroRNA in human cells. Science 309 1573-1576... 

Boiziau, C., Larrouy, B., Moreau, S., Cazenave, C., Shire, D. and Toulme, J.-J. (1992a) Ribonuclease H-mediated inhibition of translation and reverse transcription by antisense oligodeoxynucleotides. Biochem. Soc. Transactions,... 

Bolziau C, Kurfist R, Cazenave C, RoigV Thoung NT Toulme JJ. Inhibition of translation initiation by antisense oligonucleotides via an RNAase independent mechanism, Nucleic Acid Res 1991 I 9 1 I 13-1 I 19. 

An in vitro reticulocyte translation assay (Boehringer Mannheim) was modified to determine an inhibition of translation by alkaloids. An assay (total volume 25 jl) contained 2 jl 12.5 x translation mix (Boehringer), 10 pi reticulocyte lysate, 200 mM K-acetate, 1.5 mM Mg-acetate, 0.25 pCi L-[4,5-3H(N)]-leucine, 0.5 pg TMV-RNA (Boehringer) and up to 5 mM alkaloids (buffered to pH 7). The mixture was incubated at 30 °C reactions were terminated after 0, 10, 20, 30 and 40 min. The radiolabeled protein was precipitated by adding 200 pi ice-cold trichloroacetic acid (TCA) (50% w/v) and, after 30 min, filtered through GF 34 filters (Schleicher-Schull), which binds proteins. After washing the filters three times with 50% TCA, they were dried at 85 °C. Radioactivity of the filters was determined in a liquid scintillation counter.19... 

Inhibition of RNA polymerase, intercalation into DNA Inhibition of translation DNA binding and cleavage Inhibition of translation... 

Inhibition of l-phosphatidylinositol-4,5-biphosphate phosphodiesterase Inhibition of translation Inhibition of DNA topoisomerase... 

Kebache, S., Cardin, E., Nguyen, D.X, Chevet, E. and Larose, L. (2004) Nck-1 antagonizes the endoplasmic reticulum stress-induced inhibition of translation. J. Biol. Chem. 279, 9662-9671. 

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