Induced Binding

Crotonoside. Crotonoside, also caHed isoguanosine, (45) has been isolated from Croton tiglium (4). Ip administration of [2- C]crotonoside to rats results in incorporation into nucleic acids (qv). It inhibits the inducible binding sites, inhibits glutamic acid dehydrogenase, and accumulates cAMP. 

Fig. 6-12. Different types of binding sites in polymers containing micro- (site B), meso- and macropores (site A) C) Embedded site, D) Site complementary to dimer or multimer, E) Induced binding site, F) Nonselective site, G) Residual template. 

Yamamoto, K., Gonzalez, G., Biggs, W. and Montminy, M. Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB. Nature 334 494- 98, 1988. 

When upstream signaling transduction mechanisms contributing to DON-medi-ated apoptosis were investigated in cloned macrophages, specific inhibition of PKR and Hck additively suppressed DON-induced caspase-3 activity and apoptosis as well as p38, ERK, and JNK phosphorylation.59 Inhibition of PKR and Hck also inhibits DON-induced binding and enzyme activity of p53. 

Figure 9.11 Promoter-induced binding energy shifts of Ar, Kr and Xe photoemission peaks with respect to adsorption on the clean metal as a function of the distance of the adsorption site to the nearest potassium atom on a potassium-promoted Rh( 111) surface. These curves reflect the variation of the surface potential (or local work function) around an adsorbed potassium atom. Note the strong and distance-dependent local work function at short distances and the constant local work function, which is lower than that of clean Rh( 111) at larger distances from potassium. The lowering at larger distances depends on the potassium coverage. The averaged distances between the potassium atoms are 1.61, 1.32 and 1.20 nm for coverages of 2.7, 4.1 and 5.0% respectively, vertical lines mark the half-way distances. Lines are drawn as a guide to the eye (adapted from Janssens et al. [38]).

Ohlsson, B.C., Englund, M.C., Karlsson, A.L., Knutsen, E., Erixon, C, Skribeck, H., Liu, Y., Bondjers, G., and Wiklund, O., 1996, Oxidized low density lipoprotein inhibits hpopolysaccharide-induced binding of nuclear factor-kappaB to DNA and the subsequent expression of tumor necrosis factor-alpha and interleukin-1 beta in macrophages, J. Clin. Invest. 98 78-89. 

Fig. 12. Induced binding of a guest in 23 by the addition of a metal ion. (Reproduced with permission from the American Chemical Society)...

Yamamoto O (1973) Radiation-induced binding of nucleic acid constituents with protein constituents and with each other. Int J Radiat Phys Chem 5 213-229 Yamamoto O, Mandal PC (1988) Radiation-induced fluorescence changes of 2 -deoxycytidine and cytidilyl-cytidine in aqueous solution. Comparison with radiolytic behaviors of other nudeo-base derivatives. J Radiat Res 131-143... 

Induced binding capacity to hepatic microsomal protein ( mol/liver) — — 175 — 85... 

The lacl gene has its own promoter (Pkd) to which RNA polymerase binds and initiates transcription. In the absence of an inducer, the lacl gene is transcribed, producing lac repressor mRNA and hence lac repressor protein monomers. These monomers assemble to form active tetramers which bind to the lac operator site, 0, , and prevent transcription of the lac operon. In the presence of an inducer (such as allolactose or IPTG), the inducer binds to the repressor and changes its conformation, reducing its affinity for the lac operator. Thus the repressor now dissociates and allows RNA polymerase to transcribe the lac operon. 

Fig. 9.n Promoter-induced binding energy shifts of Ar, Kr and Xe photoemission peaks with respect to adsorption on the clean metal as a function of the distance of the adsorption site to the nearest potassium atom on a potassium-promoted Rh(lll) surface. These curves reflect the variation of the surface potential (or local work function) around an adsorbed potassium atom. Note the strong and distance-dependent local work function... 

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