Micronucleus assay in vitro

In vitro mammalian cell assay chromosome aberrations or tk mutations in mouse lymphoma cells 10 mM In vitro mammalian cell assay chromosome aberrations or tk mutations in mouse lymphoma cells or in vitro micronucleus assay 1 mM or 0.5 mg/ml top concentration No in vitro mammalian assay... 

Diaz, D., Scott, A., Carmichael, P Shi, W. and Costales, C. (2007) An assessment of the performance of an automated scoring system (Cellomics) for the in vitro micronucleus assay in CHO-Kl cells. Mutation Research, 630, 1-13. 

Bryce, S.M., Avlasevich, S.L., Bemis, J.C., Lukamowicz, M Elhajouji, A., Van Goethem, F., De Boeck, M Beerens, D. et al. (2008) Interlaboratory evaluation of a flow cytometric, high content in vitro micronucleus assay. Mutation Research, 650, 181-195. 

Figure 2-4 Automated image analysis of the in vitro micronucleus assay. See color plates.

Bryce SM, Bemis JC, Avlasevich SL, et al. In vitro micronucleus assay scored by flow cytometry provides a comprehensive evaluation of cytogenetic damage and cytotoxicity. Mutat Res. 2007 630(l-2) 78-91. 

Kirsch-Volders M, Sofuni T, Aardema M, Albertini S, Eastmond D, Fenech M, et al. Report from the in vitro micronucleus assay working group. Mutat Res 2003 540 153-63. 

Parry JM, Parry EM. The use of the in vitro micronucleus assay to detect and assess the aneugenic activity of chemicals. Mutat Res 2006 607 5-8. 

Westerink WM, Schirris TJ, Horbach GJ, Schoonen WG (2011) Development and validation of a high-content screening in vitro micronucleus assay in CHO-kl and HepG2 cells. Mutat Res 724 7-21... 

Kirsch-Volders, M., Sofuni, T., Aardema, M., Albertini, S., Eastmond, D., Fenech, M., Ishidate, M., Jr., Lorge, E., Norppa, H., Surralles, J., von der Hude, W., and Wakata, A. (2000). Report from the in vitro micronucleus assay working group. Environ Mol Mutagen 35, 167-172. 

Figure 10.1. Correlation data for the sensitivity and specificity for single in vitro genotoxicity assays and assay combinations. Ames, Salmonella reverse mutation test introduced by B. Ames MLA, tk assay using the L5178Y mouse lymphoma cell line Cab, chromosome aberration test with different mammalian cell types MN, in vitro micronucleus assay with different mammalian cell types. [Data according to Kirkland et al. (2005a).]...

Figure 11.6. The basis for the in vitro micronucleus assay using cytochalasin B.

Snyder R. D. and Arnone M. R., Putative identification of functional interactions between DNA intercalating agents and topoisomerase 11 using the V79 in vitro micronucleus assay, Mutat. Res., 503, 21-35, 2002. 

---