In-Vitro Biodegradation

Ghandehari, H., Kopeckova, P., and Kopeck, J., In vitro biodegradation of pH-sensitive hydrogels containing aromatic azo bonds. Biomaterials, 18 861-872 (1997). 

Jaurand MC, Gaudichet A, Halpem S, et al. 1984. In vitro biodegradation of chrysotile fibers by alveolar macrophages and mesothelial cells in culture Comparison with a pH effect. Br J Ind Med 41 389-395. 

Samyn C, Kalala W, van den Mooter G, et al. Synthesis and in vitro biodegradation of poly (ester-ester) azo polymers designed for colon targeting, hit J Hiarm 1995 121 211-216. 

Muller, R.H. and Olbrich, C., Solid lipid nanoparticles Phagocytic uptake, in vitro cytotoxicity and in vitro biodegradation, Pharm. Ind., 61, 462, 1999. 

In order to prepare PLA nanocomposites with highly dispersed cellulose nanocrystals and porous PLA-based scaffolds with enhanced mechanical properties and thermal stability, CNCs have been incorporated into PLA fibres by electrospinning method. Fibrous biocomposite mats consisting of PLA and CNCs have been electrospun from solvent or solvent mixtures such as l,l,l,3,3,3-hexafluoro-2-propanol (HFP), DMF/chloro-form and DMF/tetrahydrofuran (THF). The electrospun PLA/CNCs bionanocomposites have demonstrated rapid in vitro biodegradability and cytocompatible properties, and could be potentially suitable in tissue engineering. ... 

Fig. 2 In vitro biodegradation of an 83 % porosity mesoporous silicon membrane...

Biocompatibility 1 Biodegradability 1 Biodegradable materials 1-2 Biodegradation kinetics 3 In-vitro biodegradation 1-2 In-vivo biodegradation 2-3 Mesoporous silicon 1... 

Pan Z-H, Jiang P-P (2007) Characteristics and in vitro biodegradation of chitosan fibers modified with gelatin. J Qin Rehabil Tissue Eng Res 11 6169-6172... 

For in vitro biodegradation studies, the materials were incubated in test tubes filled with 10 mL of 0.025 M phosphate buffer solution (pH = 7.4) at 70°C for 21 days. At regular time intervals the materials were removed from the buffer and rinsed with distilled water, then placed into incubator at 70°C for 3 h, and finally weighed within 0.001 g. 

Experiments to test in vitro biodegradation of fibers or films are very common, and these are often the first tests done to assess the biodegradability... 

Atkins, T.W. and Peacock, S.J. (1996) In vitro biodegradation of poly(beta-hydroxybutyrate-hydroxyvalerate) microspheres exposed to Hanks buffer, newborn calf serum, pancreatin and synthetic gastric juice, J. Biomater. Sci. Polym. 7, 1075-1084. 

Zhang F, Fiu 1, Wu Q, Lin X (2009) Design and in vitro biodegradation of novel hepatocyte-targetable (galactose polycation/hemoglobin) multilayers and microcapsules. Macromol Chem Phys 210 1052-1060... 

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