Immunosorbent Electron Microscopy

Roberts, I. M. and Harrison, B. D. (1979) Detection of potato leafroll and potato mop-top vimses by immunosorbent electron microscopy. Ann. Appl. Biol. 93, 289-297. 

Figure 2 General strategies for ELISA routine testing of seeds for virus, illustrated for lettuce mosaic virus (LMV) in lettuce and pea early-browning virus (PEBV) and pea seed-borne mosaic virus (PSbMV) in pea. Schemes Include the estimation of the percentage of Infected seeds with the most probable number (MPN) method, confirmation of doubtful results by Immunosorbent electron microscopy (ISEM) and check on actual seed transmission of the virus to seedlings ...

Several experimental methods can be used for the identification or mapping of epitopes. X-ray crystallography, nuclear magnetic resonance, and electron microscopy map the structural epitopes that are in contact with antibody, whereas methods such as PEPSCAN and enzyme-linked immunosorbent assay are functional in approach (6). These experimental approaches, like others, require resources, time, and money. 

The myelin formation is most often studied using microscopic analysis (including electron microscopy) of the immunocytochem-istry for specific proteins, such as myelin basic protein (MBP), protein zero, myelin associated glycoprotein (MAG), and peripheral myelin protein 22 [107, 108]. The same markers can also be evaluated by other protein assays (e.g., enzyme-linked immunosorbent assay (ELISA) and western blot) or at mRNA level using for example PCR or microarray assays. 

Although iron is essential for normal functioning of the human body, it may aiso play a deleterious role as a trigger of various diseases [ 1,2]. In this chapter, we will present results of studies on the role of iron in neurodegenerative diseases such as Parkinson s disease (PD), Alzheimer s disease (AD), and progressive supranuclear palsy (PSP). We report on studies whose main tool was Mossbauer spectroscopy, but also other complementary techniques such as electron microscopy, enzyme-linked immunosorbent assay (ELISA), and atomic absorption. 

Methods BF = bright field LM of tissue impression smears, wet-mounts, stained whole mounts LM = light microscopy PH = phase microscopy DF = dark-field microscopy TEM/SEM = transmission/scanning electron microscopy of sections or of purified or semi-purified virus ELISA = enzyme-linked immunosorbent assay PAb = polyclonal antibodies MAb = monoclonal antibodies DBH = dot blot hybridization ISH = in situ hybridization PCR = polymerase chain reaction RT-PCR = reverse transcription PCR. 

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