Polysaccharide immunogen preparation

In preparation for use as a surrogate vaccine, these peptides were synthesized as conjugates to tetanus toxoid, and in this form also competed with the 0-antigen polysaccharide for binding [104]. Whether these peptides will function as effective immunogenic mimics will likely depend on their propensity to adopt the bioactive conformation in solution. A certain amount... 

Hydrophobically modified polymers can associate in aqueous media to form micelle-like structures above their critical association concentrations (CACs). The nanosized self-aggregates were prepared using modified natural polysaccharides such as pullulan, curdlan, and glycol chitosan. The modified polysaccharides provide excellent biocompatibility, biodegradability, low immunogenicity, and biological activities. 

Schneerson R, Barrera O, Sutton A, Robbins JB. Preparation, characterization, and immunogenicity of Haemophilus influenzae type b polysaccharide-protein conjugates. J Exp Med 1980 152 361-376. 

For an immunological characterization monoclonal antibodies were produced using a polysaccharide from cotyledons as immunogen. Positive hybridoma cell lines cross-reacted with various polysaccharide preparations, including those from pea and spinach leaves. However, no cross-reaction with homoglucans, such as glycogen or starch, was observed (Fig. 3). 

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