Immobilized Artificial Membrane chromatography

Pidgeon, C., Venkataram, U. V. Immobilized artificial membrane chromatography supports composed of membrane lipids. Anal. Eiochem. 1989,... 

Ong, S. Liu, H. Pidgeon, C., Immobilized-artificial-membrane chromatography Measurements of membrane partition coefficient and predicting drug membrane permeability, J. Chromatogr. A 728, 113-128 (1996). 

This agrees to internal VolSurf models derived for PAMPA membrane transport [163] to understand passive transcellular transport across membranes. One of our internal models based on 29 compounds characterized by immobilized artificial membrane chromatography by Salminen etal. ]164] shows an of 0.81 and = 0.70 for two PLS components derived using VolSurf descriptors. This is one of the rare examples where ionized starting molecules led to slightly better PLS statistics, while the general chemical interpretation is not affected. 

JA Masucci, GW Caldwell, JP Foley. Comparison of the retention behavior of beta-blockers using immobilized artificial membrane chromatography and ly-sophospholipid micellar electrokinetic chromatography. J. Chromatogr. A 810 95-103 (1998). 

Stewart BH, Chan OH (1998) Use of immobilized artificial membrane chromatography for drug transport applications. J Pharm Sci 87 1471-1478 Taillardat-Bertschinger A, Carrupt PA, Barbato F et al. (2003) Immobilized artificial membrane HPLC in drug research. J Med Chem 46 655-665... 

The use of partition coefficients between water and lipophilic media is of wide use in pharmaceutical research. As discussed in the last chapters, different lipophilicity scales are used to describe the lipophilicity of a compound and relate it to its absorption behaviour in vivo. Differences between the logPow and partitioning between phospholipids and water (mainly determined using liposomes) for diverse compounds have been described leading to the development of the immobilized artificial membrane chromatography system. However, also the predictivity of the IAM system is limited and only a small number of membrane systems are available. 

Chromatography also can be used to predict permeability. The most widely used methods are immobilized artificial membrane chromatography (lAM) and immobilized liposome chromatography (ILC). 

Pehourcq, F., Jarry, C. and Bannwarth, B. (2003) Potential of immobilized artificial membrane chromatography for lipophilicity determination of arylpropionic acid non-steroidal antiinflammatory drugs. Journal of Pharmaceutical and Biomedical Analysis,... 

BH Stewart, OH Chan. Use of immobilized artificial membrane chromatography for drug transport applications. J Pharm Sci 87 1471—1478, 1998. 

Chan EC, Tan WL, Ho PC, Fang LJ. Modeling Caco-2 permeability of drugs using immobilized artificial membrane chromatography and physicochemical descriptors. J Chromatogr A 2005, 29 Apr 1072(2) 159-168. 

Cohen DE and Leonard MR (1995) Immobilized artificial membrane chromatography a rapid and accurate ITPLC method for predicting bile salt-membrane interaction. J Lipid Res 36 2251 2260. 

Ollila et al. (2002) studied the interaction between flavonoids and membranes composed of di-palmitoylphosphatidylcholine by means of nonco-valent immobilized artificial membrane chromatography and flavonoid-induced calcein release from fluid egg phosphatidylcholine vesicles. Flavonoids with more hydroxyl groups showed longer retention delays in the immobilized artificial membrane studies, suggesting stronger interactions between the flavonoids, which are rich in hydroxyl groups, and the dipalmitoylphosphatidylcholine membrane interface. Both polar and nonpolar forces were shown to have a significant impact on the flavonoid-biomembrane interactions. 

Cai SJ, Me Andrew RS, Leonard BP, Chapman KD, Pidgeon C. (1995). Rapid purification of cottonseed membrane-bound N-Acylphosphatidylethanolamine synthase by immobilized artificial membrane chromatography. J Chromatography 696 49-62. Chapman KD, Lin I, Decease AD. (1995). Metabolism of cottonseed microsomal A -Acylphosphatidylethanolamine. Arch Biochem Biophys 318 401-407. 

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