Immobilized liposomes

Lundahl, P., Beigi, F. Immobilized liposome chromatography of drugs for model analysis of drug-membrane interactions. Adv. Drug Deliv. Rev. 1997, 23, 221-227. 

Immobilized liposome chromatography (ILC) is an (albeit related) alternative to IAM [96, 97]. Compounds with the same log P were shown to have very different degrees of membrane partitioning on ILC, depending on the charge of the compound [97]. 

Yang, Q., Liu, X. Y., Umetani, K., Kamo, N. and Miyake, J. (1999). Partitioning of triphenylalkylphosphonium homologues in gel bead-immobilized liposomes chromatographic measurement of their membrane partition coefficients, Biochim. Biophys. Acta-Biomem., 1417, 122-130. 

Tatsu et a/.(106) reported a novel immunosensor using immobilized liposomes doped with carboxyfluorescein and dinitrophenyl (DNP) hapten on the tip of an optical fiber. On complement-mediated immunolysis by anti-DNP-antibody, the fluorescent signal of the liberated carboxyfluorescein was measured. 

ILC, immobilized liposome chromatography MEKC, micellar electrokinetic capillary chromatography. 

C Lagerquist, F Beigi, A Karlen, H Lennernas, P Lundahl. Effects of cholesterol and model transmembrane proteins on drug partitioning into lipid bilayers as analyzed by immobilized-liposome chromatography. J Pharm Pharmacol 53 1477-1487, 2001. 

F Beigi, Q Yang, P Lundahl. Immobilized-liposome chromatographic analysis of drug partitioning into lipid bilayers. J Chromatogr A 704 315-321, 1995. 

E Brekkan, L Lu, P Lundahl. Properties of immobilized-liposome-chromat-ographic supports for interaction analysis. J Chromatogr A 711 33—42, 1995. 

X-Y Liu, Q Yang, N Kamo, J Miyake. Effect of liposome type and membrane fluidity on drug-membrane partitioning analyzed by immobilized liposome chromatography. J Chromatogr A 913 123-131, 2001. 

X-Y Liu, Q Yang, C Nakamura, J Miyake. Avidin-biotin-immobilized liposome column for chromatographic fluorescence on-line analysis of solute-membrane interactions. J Chromatogr B 750 51-60, 2001. 

Falten et al. recently reported that phospholipid membrane vesicles can be con-stmcted on a filter scaffold without any organic solvent [57-60]. In this system, the phospholipid vesicle occupies the filter pores to form a permeation barrier. This is more relevant to the cellular membrane than PAMPA membranes with organic solvent. The membrane can be stored up to two weeks without significant change and is stable at pH 2-8. The Fa% predictability was compared with BM-PAMPA, DS-PAMPA, Caco-2 and immobilized liposome chromatography, resulting in promising predictability. 

Vermette P, Meagher L, Gagnon E et al (2002) Immobilized liposome layers for drug delivery applications inhibition of angiogenesis. J Control Release 80 179-195... 

The partition coefficients of triphenylalkylphosphonium homologs have been determined in gel bead-immobilized small or large unilamellar liposomes by chromatography [8]. It was claimed that the technique, immobilized liposome chromatography (ILC), is suitable for the determination of membrane partition coefficients of drugs. 

Several studies have been undertaken to measure the retention times of compounds on chromatography columns comprising immobilized liposomes or artificial membranes. Liposomes comprise aqueous compartments surrounded by lipid bilayers, whereas the artificial membranes comprise monolayers of phospholipids (Lundahl and Beigi, 1997). Interaction between the compound and the phospholipids present mimics the interaction of compounds with cell membranes. Retention... 

Chromatography also can be used to predict permeability. The most widely used methods are immobilized artificial membrane chromatography (lAM) and immobilized liposome chromatography (ILC). 

---