Imaging luminescent bioprobes

Ligands Near-Infrared Materials Lanthanides Magnetic Resonance Imaging Luminescent Bioprobes Sensors for Lanthanides and Actinides. 

Sergeeva N. N., M. Donnier-Marechal, G. Vaz, A. M. Davies, M. O. Senge. Synthesis and evaluation of the europium(III) and zinc(II) complexes as luminescent bioprobes in high content cell-imaging analysis, J. Inorg. Biochem., 105, 1589 1595 (2011). 

Deiters E, Song B, Chauvin AS, Vandevyver CDB, Gumy F, Bunzli JCG (2009) Luminescent bimetallic lanthanide bioprobes for cellular imaging with excitation in the visible-light range. Chem Eur J 15 885-900... 

Figure 13.27 (Top) Luminescence images of HeLa cells loaded with different concentrations of [Eu2(L62)3] in RPMI-1640 for 7h at 37°C. (Lex = 330nm, Xem >585nm, exposure time 60s). (Middle) Images of HeLa cells loaded with 250 p.M [Eu2(L62)3] (5h at 37°C, exposure time 10 s), then incubated with 40mgmL acridine orange (Xex = 450 90 nm Xem = 515-565 nm, exposure time 10 ms) in PBS (5 min at room temperature). (Bottom) Co-localization experiments cells loaded with 250 p.M [Eu2(L62)3] and 15 mgmL BIODIPY PL LDL (0.5 h, Xex = 470 nm, 2 s exposure time) [77]. (Reproduced from E. Deiters et al., Effect of the length of polyoxyethylene substituents on luminescent bimetallic lanthanide bioprobes, New Journal of Chemistry, 32, 1140-1152, 2008, by permission of The Royal Society of Chemistry (RSC) for the Centre National de la Recherche Scientifique (CNRS) and the RSC.)...

Deiters, E., Song, B., Chauvin, A.S., et al. (2009) Luminescent bimetallic lanthanide bioprobes for cellular imaging with excitation in the visible-Ught range. Chemistry —A European Journal, 15, 885—900. Charbonniere, L.J., Weibel, N., Estoumes, C., et al. (2004) Spatial and temporal discrimination of siUca particles functionalized with luminescent lanthanide markers using time-resolved luminescence microscopy. New Journal of Chemistry, 28, 777—781. 

Deiters E., B. Song, A.-S. Chauvin, C. Vandevyver, J.-C. G. BunzU. Luminescent BimetaUic Lanthanide Bioprobes for CeUular Imaging with Excitation into the Visible, Chem. Eur. J., 15, 885-900 (2009). 

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