Luminescence bioprobes

Biinzli JG (2009) Lanthanide luminescent bioprobes (LLBs). Chem Lett 38 104—109... 

Deiters E, Song B, Chauvin AS, Vandevyver CDB, Gumy F, Bunzli JCG (2009) Luminescent bimetallic lanthanide bioprobes for cellular imaging with excitation in the visible-light range. Chem Eur J 15 885-900... 

Figure 13.27 (Top) Luminescence images of HeLa cells loaded with different concentrations of [Eu2(L62)3] in RPMI-1640 for 7h at 37°C. (Lex = 330nm, Xem >585nm, exposure time 60s). (Middle) Images of HeLa cells loaded with 250 p.M [Eu2(L62)3] (5h at 37°C, exposure time 10 s), then incubated with 40mgmL acridine orange (Xex = 450 90 nm Xem = 515-565 nm, exposure time 10 ms) in PBS (5 min at room temperature). (Bottom) Co-localization experiments cells loaded with 250 p.M [Eu2(L62)3] and 15 mgmL BIODIPY PL LDL (0.5 h, Xex = 470 nm, 2 s exposure time) [77]. (Reproduced from E. Deiters et al., Effect of the length of polyoxyethylene substituents on luminescent bimetallic lanthanide bioprobes, New Journal of Chemistry, 32, 1140-1152, 2008, by permission of The Royal Society of Chemistry (RSC) for the Centre National de la Recherche Scientifique (CNRS) and the RSC.)...

Extension to the use of multi-photon induced luminescence lanthanide-based bioprobes adds new possibilities and challenges to the field. However, there are even fewer examples of multiphoton lanthanide bioprobes because achieving acceptable quantum yields is fairly difficult in view of the numerous nonradiative deactivation pathways created by a wealth of vibrations, including high energy oscillators located far from the emitting lanthanide ion. 

Deiters, E., Song, B., Chauvin, A.S., et al. (2008) Effect of the length of polyoxyethylene substiments on luminescent bimetallic lanthanide bioprobes. New Journal of Chemistry, 32, 1140-1152. 

Deiters, E., Song, B., Chauvin, A.S., et al. (2009) Luminescent bimetallic lanthanide bioprobes for cellular imaging with excitation in the visible-Ught range. Chemistry —A European Journal, 15, 885—900. Charbonniere, L.J., Weibel, N., Estoumes, C., et al. (2004) Spatial and temporal discrimination of siUca particles functionalized with luminescent lanthanide markers using time-resolved luminescence microscopy. New Journal of Chemistry, 28, 777—781. 

Elhabiri et al., 2004a). The ultimate fixation of the third ligand in the neutral helicate [f 2(L13%] induces a considerable entropic gain to the overall complexation process, which is responsible for both extreme thermodynamic stability (Elhabiri et al., 1999) and kinetic inertness (Elhabiri et al., 2004b, Figure 59), which make these helicates promising building blocks for the development of luminescent bioprobes (see Section 6). 

The structural studies by X-ray diffraction and NMR have been complemented by a high-resolution luminescence analysis of the Eu "-containing complexes similar to the one conducted for the homometallic bioprobes (Section 6.3.1). Data are collected in Table 24. Firstly, the energy of the Dq —> transition calculated with Eq. (19) (see Section 3.5) and the... 

Shen J, Sun LD, Yan CH (2008) Luminescent rare earth nanomaterials for bioprobe applications. Dalton Trans 5687-5697... 

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