Imaging immune complexes

Both tips were transferred into laboratory ambient. The active tip was placed in buffer containing ferritin/IgG conjugate at a concentration of about 12.5 micrograms/ml. After three minutes the active tip was rinsed as described in (3), above. Previous TEM images confirmed that this procedure resulted in a saturation coverage of the immune complex on the tip surface. The control tip remained in laboratory ambient during the deposition procedure. 

Kim DM, Tien R, Bymm C, Ki islinan KR (1996) Imaging in acquired immune deficiency syndrome demenda complex (AIDS demenda complex) A review. Prog Neui opsychophannacol Biol Psychia-tiy 20 349-370. 

Some studies have attempted direct injection of DNA into tumors, either alone or in complex with lipids. Introducing DNA or RNA directly into the tumor limits immune responses sometimes elicited when viral preparations are administered. However, the efficiency of lipid-DNA to enter cells in a tumor remains a problem, as does the relatively nonspecific cellular uptake of lipid-DNA complexes. Attempts to incorporate molecules in the lipid bilayer that bind to cell surface receptor to improve cell specificity have been investigated, but the differential effects seen in vitro are not generally duplicated in vivo. Along the same line and more recently, nanoparticles has also been considered as vector for delivering therapeutic genes. An added benefit with nanoparticles is that they can also be imaged (Nie et al. 2007). 

Gombos 1, Steinbach G, Pomozi 1, et al. Some new faces of membrane microdomains a complex confocal fluorescence, differential polarization, and PCS imaging study on live immune cells. Cytometry A. 2008 73(3) 220-229. 

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