IEC-18 cell

Duizer, E., Penninks, A. H., Stenhuis, W. H., Groten, J. P., Comparison of permeability characteristics of the human colonic Caco-2 and rat small intestinal IEC-18 cell lines, J. Controlled Release 1997,... 

Duizer, E., et al. 1998. Absorption enhancement, structural changes in tight junctions and cytotoxicity caused by palmitoyl carnitine in Caco-2 and IEC-18 cells. J Pharmacol Exp Ther 287 395. 

Another human colonic cancer cell line is T84, which forms monolayers that are even tighter than those of the Caco-2. It has been described as resembling a colonic crypt cell phenotype. Hence, these cells have been used mainly in studies of epithelial ion secretion and are generally not considered to be adequate for drug transport studies, particularly with respect to carrier-mediated processes [13, 91, 92]. The rat intestinal epithelial cell line IEC-18 has been evaluated as a model to study small intestinal epithelial permeability. This cell line, which forms very leaky monolayers, was proposed to be a better model than the Caco-2 monolayers for evaluating the small intestinal paracellular permeation of hydrophilic molecules [93]. Importantly, the leaky tight junctions of the IEC-18 cells are a result of an undeveloped paracellular barrier lacking the perijunctional actin belt. In addition, the IEC-18 cells have minute expression of transporters [91, 93]. 

Production of Mucosal Damage 2.3.1.2.1 Cell culture Stimulated neutrophils are known to be cytotoxic to cells in vitro (Dull et al., 1987 Dallegri et al., 1990 Grisham et al., 1990b). Several in vitro systems have been used to demonstrate oxidative damage to intestinal cells. Xanthine/XO increased Cr release and decreased [ H]thymidine uptake by IEC-18 small intestinal epithelial cell monolayers in a dose-dependent manner (Ma et al., 1991). Rat enterocytes show decreased trypan blue exclusion and increased protein release when incubated with neutrophils stimulated... 

Ma, T.Y., Hollander, D., Freeman, D., Nguyen, T. and Krugliak, P. (1991). Oxygen free radical injury of IEC-18 small intestinal epithelial cell monolayers. Gastroenterology 100, 1533-1543. 

Ma, T., D. Hollander, D. Bhalla, H. Nguyen and P. Krugliak (1992). IEC-18, a nontrans-formed small intestinal cell line for studying epithelial permeability. J Lab Clin Med 120 329-341. 

Duizer E, Stenhuis WS, Penninks AH, Groten JP (1995) Transport of compounds across monolayers of intestinal cell lines comparison of IEC-18 and CACO-2. Ital J Gastroenterol 27 154... 

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