Hydrogen peroxidase

Fig. 22.2. Schematic representation of the enzymatic process involving paracetamol and hydrogen peroxidase catalyzed by peroxidase of the zucchini tissue powder incorporated into the Nujol/graphite electrode.

Substrate one step TMB (Zymed) is a common choice see Note 4). Although many other substrates are available for HRP, TMB has high sensitivity with a quick development time. OD can be monitored at 650 nm as the color develops, then at 450 nm, when the reaction is stopped with H2S04. TMB may also be obtained in a lyophilized state and made up fresh with hydrogen peroxidase or as a preprepared one-step solution. TMB can vary considerably between different manufacturers, and this can affect the sensitivity and specificity of the ELISA. With one-step TMB, there is often large batch-to-batch variation as well. Each batch therefore needs to be tested before use. 

The hydrophobias are a case where protein nanofibers can play a dual role in creating a biosensor. They can aid in the immobilization of bioactive components within a biosensor and also add further functionality to the transducing element of a biosensor device. Hydrophobins are self-assembling [3-sheet structures observed on the hyphae of filamentous fungi. They are surface active and aid the adhesion of hyphae to hydrophobic surfaces (Corvis et al., 2005). These properties can be used to create hydrophobia layers on glass electrodes. These layers can then facilitate the adsorption of two model enzymes glucose oxidase (GOX) and hydrogen peroxidase (HRP) to the electrode surface. The hydrophobin layer also enhances the electrochemical properties of the electrodes. 

The accumulation of hydrogen peroxidase affects many intracellular processes and results in hemolysis. These include the cross-linking of membrane proteins hemoglobin denaturation (manifest as Heinz body formation), which in turn affects the physical properties of the erythrocyte and lipid peroxidation, which may affect the cell membrane to cause direct hemolysis (Fig. 11-8). The resultant damage leads to a mixture of intravascular hemolysis and extravascu-lar hemolysis (by which hemolysis occurs in the reticuloendothelial system). In acute hemolytic episodes, the clinical picture is of predominantly intravascular hemolysis, while predominantly extravascular hemolysis is seen in patients with chronic hemolysis. 

Glutathione hydrogen-peroxidase oxidoreductase (EC 1.11.1.9) is a selenoprotein enzyme that catalyses the reduction of a large number of hydroperoxides ranging from H202 to a spectrum of organic hydroperoxides (ROOH). 

Immunocytochemistry The presence of the labeled probe bound to the in situ nucleic acid target is detected by means that relate specifically to the nature of the label. The most frequently used labels are enzymes (alkaline phosphatase, hydrogen peroxidase) and fluorochromes (fluorescein, rhodamine, hydroxycoumarin). 

Metallic mercury can be oxidized to inorganic divalent mercury by the hydrogen peroxidase-catalase pathway, which is present in most tissues. The inorganic divalent cation can, in turn, be reduced to... 

Zirconia nanopartides enhanced grafted collagen tri-hehx scaffold for unmediated biosensing of hydrogen peroxidase. Lar muir, 22, 8915 19. 

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