Human serum lipoproteins

H. Kirn and W. C. Galley, Rotational mobility associated with the protein moiety of human serum lipoproteins from tryptophan phosphorescence anisotropy measurements, Can. J. Biochem. Cell Biol. 61, 46-53 (1983). 

Human Serum Lipoproteins Percentage Protein Lipid Distribution"... 

H3. Havel, R. J., Shore, V. G., Shore, B., and Bier, D. M., Role of specific glyco-peptides of human serum lipoproteins in the activation of lipoprotein lipase. Circ. Res. 27, 595-600 (1970). 

M4. Margolis, S., Separation and size determination of human serum lipoproteins by agarose gel tration. J. Lipid Res. 8, 501-507 (1967). 

N2. Nichols, A. Y., Human serum lipoproteins and their interrelationships. Advan. Biol. Med. Phys. 11, 109-158 (1967). 

Schultz, J. S., Shreffier, D. S., and Harvie, N. R., Genetic and antigenic studies and partial purification of human serum lipoprotein carrying the Lp antigenic determinant. Proe. Nat. Acad. Sd. U.S. 61, 963-970 (1968). 

Shore, B., and Shore, V., Isolation and characterization of polypeptides of human serum lipoproteins. Biochemistry 8, 4510-4616 (1969). 

Figure 3.11 Composition (%) of human serum lipoproteins VLDL, very low density lipoproteins LDL, low density lipoproteins HDL, high density lipoproteins.

O. Chassany, S. Urien, P. Claudepierre, G. Bastian, and J. P. Tillement, Binding of anthracycline derivatives to human serum lipoproteins, Anticancer Res. 14 2353-2355 (1994). 

G34. Gustafson, A., Alaupovic, P., and Furman, R. H., Studies of the composition and structure of serum lipoproteins Physical-chemical characterization of very low density human serum lipoproteins. Biochim. Biophys. Acta 84, 767-769 (1964). 

P19. Polz, E., and Kostner, G. M., The binding of 32-glycoProte n-Ito human serum lipoproteins. Distribution among density fractions. FEBS Lett. 102, 183-186 (1979). 

Kerckhoffs, D.A. et al., Effects on the human serum lipoprotein profile of p-glucan, soy protein and isoflavones, plant sterols and stanols, garlic and tocotrienols, J. Nutr, 132, 2494, 2002. 

Utilizing the buoyant densities listed in Table II and the frictional ratio of 1.11, values for the sedimentation coefficient have been calculated at two different solvent densities the standard S value routinely used to characterize human serum lipoproteins is defined as value of the flotation coefficient in Svedberg units (the negative sedimentation coefficient X 10 sec) in an aqueous NaCl solvent with a density of 1.063 g/ml and a viscosity of 1.021 centipoise (the viscosity of a 1.063 g/ml sodium chloride solution at 26°C). These values are listed in Table II. The S value is very sensitive to small variations in lipoprotein density because the solvent density is close to the lipoprotein density. To compare the particle sizes or molecular weights, values of the sedimentation coefficient (5) in a solvent with a density of 1.20 g/ml and the viscosity of KBr at 25°C are preferred, and the computed values are listed in Table II. 

Countercurrent chromatography is a very useful technique for the separation and fractionation of human serum lipoproteins. The HDL-LDL and VLDL-serum protein fractions were directly obtained from human serum using... 

Shore, B., C- and N-terminal amino acids of human serum lipoproteins. Arch. Biochem. Biophys. 71, 1-10 (1957). 

Schleicher, E., Deufeel, T., and Wieland, O. H., Non-enzymatic glycosylation of human serum lipoproteins elevated lysine-glycosylated low density lipoproteins in diabetic patients. FEBS Lett. 129, 1-4 (1981). 

Additional large lipid molecules have been directly analyzed by GC. Typical studies of this type include the determination of Coenzyme A esters [339], intact triacylglycerols of human serum lipoproteins [340], tocopherols as trimethylsilyl derivatives [341], and ceramides as both trimethylsilyl [342,343] and boronate [344] derivatives. 

The applicability of 13C nmr to human serum lipoproteins has been demonstrated (Hamilton et al., 1973, 1974). Spectra and partial peak assignments for high-density (HDL), low-density (LDL), and... 

Cornwell, D. G., Kruger, F. A., and Robinson, H. B. (1962). Studies on the absorption of beta-carotene and the distribution of total carotenoid in human serum lipoproteins after oral administration. J. Lipid Res. 3, 65-70. 

In view of the fact that pectin lowers serum cholesterol levels and cholesterol Is carried by lipoproteins In the serum, we investigated the Interaction of pectin obtained from grapefruit albedo with various human serum lipoprotein fractions in vitro (22). It was observed that pectin Interacted specifically with LDL, while no Interaction was observed between pectin and high density or very low density lipoproteins. The biological significance of this observed in vitro Interaction of pectin with LDL remain unclear, primarily because of lack of evidence suggesting entry of pectin or some component of It into the blood streatm. Therefore, unless such entry and/or absorption of pectin or one of Its components is demonstrated, the biological Implication of this observed Interaction... 

Any discussion of serum lipids must ultimately lead to a consideration of the serum lipoproteins since it is in the form of lipoproteins that the lipids of the serum circulate. This volume presents two authoritative discussions on the subject of lipoproteins. One chapter discusses the analysis of human serum lipoproteins and emphasizes the possibilities inherent in the application of computer techniques to such analyses. The other chapter presents a thorough discussion of lipoprotein structure, metabolism, and catabolism. It touches on all aspects of lipoprotein metabolism. 

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