Human EGF receptor

A recent example of the antibody-based approach is the discovery of a monoclonal antibody against human EGF receptor (HER2), a... 

Trastuzumab (Herceptin) [Antineeplastic/Monoover express the HERlIneu. protein breast CA adjuvant, w/ doxorubicin, cyclophosphamide, and paclitaxel if pt HER2/neu(+) Action MoAb binds human EGF receptor 2 protein HER2) mediates cellular cytotox Dose Per protocol, typical 2 mg/kg/IV/wk Caution [B, ] CV dysfxn, alla-gy/inf Rxns Contra Live vaccines Disp Inj SE Anemia, cardiomyopathy, nephrotic synd, pneumonitis Interactions t Risk of cardiac dysfxn W/ anthracycline, cyclophosphamide, doxorubicin, epirubicin EMS Cardiomyopathy, ventricular dysfxn and pulm tox have been reported monitor for Sxs of reduced cardiac Fxn OD Sxs unknown... 

Lin CR, Chen WS, Kruiger W et al. Expression cloning of human EGF receptor complementary DNA gene amplification and three related messenger RNA products in A431 cells. Science 1984 224 843-848. 

Fig. 1. A diagrammatic comparison of the structures of the human EGF receptor and the human c-erbB-2 protein. SH, cysteine-rich sequence PI, P2, P3, the three mapped sites of autophosphorylation of the EGF receptor.

Fig. 2. A comparison of the sequences surrounding the sites of phosphorylation by protein C of the human EGF receptor and the human c-src protein. Taken from Gould et al. [13]. The single-letter amino acid code is used., serine (S) or threonine (T) at equivalent positions.

PHOSPHORYLA TWN chronic myelongenous leukemia-associated KIO BCR-ABL Drosophila Kr protein hepatitis B virus core and precore antigens human c-fos protein human c-myc protein human EGF receptor human epidermal growth factor receptor (tyrosine kinase domain) human immunodeficiency virus p24 human immunodeficiency virus tat protein human insulin receptor 6-subunit human insulin receptor - tyrosine kinase domain... 

ErbB (or HER in the human). There are four ErbB receptors that form homo- or heterodimers in various combinations upon ligand binding. Specific NRG isoforms preferentially interact with different ErbB dimers. The ErbB receptors are ligand-activated tyrosine kinases structurally similar to the EGF receptor. 

Sizemore N, Choo CK, Eckert RL, and Rorke EA [1998] Transcriptional regulation of the EGF receptor promoter by HPV16 and retinoic acid in human ectocervical epithelial cells. Exp Cell Res 244 349-356... 

Panitumumab Vectibix Human IgC2K anti-EGF receptor Metastatic colorectal cancer 2006 United States... 

Vieira AV, Lamaze C, Schmid SL (1996) Control of EGF receptor signaling by clathrin-mediated endocytosis. Science 274 2086-2089 Vijay-Kumar S, Bugg CE. Wilkinson KD, Cook WJ (1985) Three-dimensional structure of ubiquitin at 28 A resolution. Proc Natl Acad Sd USA 82 3582-3585 Wang HR, Kania M, Baumeister W. Nederlof PM (1997) Import of human and Thermoplasma 20S proteasomes into nudei of HeLa cells requires functional NLS sequences. Eur J Cell Biol 73 105-113... 

Sirotnak FM, Zakowsky MF, Miller VA, et al. Potentiation of cytotoxic agents against human tumors in mice by ZD1839 (Iressa) does notrequire high levels of expression of EGF receptors. ProcAm Assoc Cancer Res 2000 41 3076 (abstr). 

Politi K, Zakowski MF, Fan PD et al. Lung adenocarcinomas induced in mice by mutant EGF receptors found in human lung cancers respond to a tyrosine kinase inhibitor or to down-regulation of the receptors. Genes Dev 2006,20 1496-1510. 

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