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HPLC high performance liquid parameters

Steuer et al. compared supercritical fluid chromatography with capillary zone electrophoresis (CZE) and high-performance liquid chromatography (HPLC) for its application in pharmaceutical analysis [24]. Efficiency, performance, sensitivity, optimization, sample preparation, ease of method development, technical capabilities, and orthogonality of the information were the parameters studied. They concluded that SFC is ideal for moderately polar compounds, such as excipients, for which mass detection is required. [Pg.391]

Body fluids are analyzed for T3 and T4 by a variety of radioimmunoassay procedures (31) (see Immunoassays). The important clinical parameter for estimating thyroid function, the protein-bound iodine (PBI), is measured as described in treatises of clinical chemistry. High performance liquid chromatographic (hplc) methods have replaced tic (32,33). [Pg.51]

The LFER-based retention parameter in high-performance liquid chromatography (HPLC) is the logarithm of the phase capacity ratio or retention factor k. The capacity... [Pg.515]

Selectivity is almost independent of pressure in high-performance liquid chromatography (HPLC) and gas chromatography (GC), whereas pressure (and corresponding density) is a very important parameter con-... [Pg.1449]

Keywords High performance liquid chromatography (HPLC), Analytical biotechnology, Sample preparation, Diode-array-detection (DAD), Mass spectrometry (MS), Validation, Preparative chromatography, Competitive isotherm parameters, Perturbation peak (PP) method... [Pg.2]

These are experimental quantities derived from chromatographic techniques, that is, from gas chromatography (GC), high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and paper chromatography (PC) [Kaliszan, 1987, 1992] or structural indices used to predict experimental chromatographic parameters from molecular structure. [Pg.136]

This extends the previous work (I ) In which the Lennard-Jones type surface potential function and the frictional function representing the Interfaclal forces working on the solute molecule from the membrane pore wall were combined with solute and solvent transport through a pore to calculate data on membrane performance such as those on solute separation and the ratio of product rate to pure water permeation rate in reverse osmosis. In the previous work (1 ) parameters Involved in the Lennard-Jones type and frictional functions were determined by a trial and error method so that the solutions in terms of solute separation and (product rate/pure water permeation rate) ratio fit the experimental data. In this paper the potential function is generated by using the experimental high performance liquid chromatography (HPLC) data in which the retention time represents the adsorption and desorption equilibrium of the solute at the solvent-polymer interface. [Pg.315]

The pharmacokinetics parameters of catechin metabolites are listed in table 7.2. Intact tea catechins, EC, ECG, EGC, and EGCG in human plasma and urine, are detected but with low concentrations. To determine the content of conjugated metabolites of catechins, blood and urine samples are treated with p-glucuronidase and sulfatase to remove the glucuonic or sulfate group prior to high-performance liquid chromatography (HPLC) analysis. [Pg.114]


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