Hormones radioimmunoassay

In light of the 27% posterior probability, the pathologist decides to order a parathyroid hormone radioimmunoassay, even though this test is expensive. If the radioimmunoassay had a sensitivity of 95% and a specificity of 98% and the results turned out to be positive, the Bayes theorem could again be used to calculate the... 

Utiger RD (1979) Thyrotropin. In Jaffe BM, Behrman HR (eds) Methods of Hormone Radioimmunoassay. Academic Press, New York, pp 315-325... 

Hemmila IA (1989) Fluorescent labels for use in fluorescence immunoassays. App Fluores Technol 1(4) 1-8 Jaffe BM, Behrman H (1979) Methods of Hormone Radioimmunoassay. Academic Press, New York Janssen MJ (1997) Develoment and perspectives of fluorescent receptor assays. A case study with benzodiazepines. http //docserver.ub.rug.n1/eldoc/dis/science/m.j.janssen Kriz D, Ramstrom O, Mosbach K (1997) Molecular imprinting. New possibilities for sensor technology. Analytical Chemistry 69 345A-349A... 

Hawker CD. Parathyroid hormone radioimmunoassay and clinical interpretation. Ann Clin Lab Sci 1975 5 383-98. 

Orth D. Adrenocorticotropic hormone (ACTH). In Jaffe BM, Behrman H, eds. Methods of hormone radioimmunoassay, 2nd ed. New York Academic Press Inc, 1979 245-83. 

Radiotracers have also been used extensively for the quantitative rnicrodeterrnination of blood semm levels of hormones (qv), proteins, neurotransmitters, and other physiologically important compounds. Radioimmunoassay, which involves the competition of a known quantity of radiolabeled tracer, usually I or H, with the unknown quantity of semm component for binding to a specific antibody that has been raised against the component to be deterrnined, is used in the rnicro deterrnination of physiologically active materials in biological samples (see Immunoassay). 

Arnaud, C. D. Brewer, H. B. Jr. "Parathyroid Hormone Structure and Immunoheterogenelty" In Methods In Radioimmunoassay, Toxicology and Related Areas". Progress In Analytical Chemistry, pp. 45-75, Vol. 8, Simmons, I. L. and Ewing, 6. W., Editors, Plenum Press, New York and London, 1974. 

S.M. Hsu, L. Raine, and H. Fanger, A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am. J. Clin. Pathol 75, 734-738 (1981). 

Henderson SR, Baker C, Fink G (1977) Oestradiol-17beta and pituitary responsiveness to luteinizing hormone releasing factor in the rat a study using rectangular pulses of oestradiol-17 beta monitored by non-chromatographic radioimmunoassay. J Endocrinol 73 441-453... 

The scope of applicability of radioimmunoassay is rapidly expanding with the dawn of each day as RIA is being developed for newer pharmaceutical substances. It has attained wide recognition and application both in vitro and in vivo measurements of compounds of interest like insulin, gastrin, glucagon, and growth hormones on one hand whereas drugs like ... 

Increased TBG leading to increased circulating total thyroid hormone, as measured by PBI, T4 by column, or T4 by radioimmunoassay. Free T3 resin uptake is... 

Felber, J. P. Radioimmunoassay of polypeptide hormones and enzymes, in Methods of Biochemical Analysis (ed.) Glick, D.,, Vol 22, p. 1., New York—London—Sydney—Toronto, John Wiley Sons, Inc. 1974... 

Use of hormonal-type substances for growth promotion and fattening purposes in food-producing animals is prohibited or restricted in many countries. Administration of diethylstilbestrol, in particular, has been totally banned worldwide. This implies that adequate analytical methods should be available for regulatory control. Due to its sensitivity, radioimmunoassay has have become most important in diethylstilbestrol analysis and represents the final detection step in the EU reference method for stilbene residues analysis (100). 

For their work on hypothalamic hormones, Schally and Guillemin shared the Nobel Prize in Physiology or Medicine in 1977, along with Rosalyn Yalow, who (with Solomon A. Berson) developed the extraordinarily sensitive radioimmunoassay (RIA) for peptide hormones and used it to study hormone action. RIA revolutionized hormone research by making possible the rapid, quantitative, and specific measurement of hormones in minute amounts. 

Hormone-specific antibodies are the key to the radioimmunoassay. Purified hormone, injected into rabbits, elicits antibodies that bind to that hormone with very high affinity and specificity. When a constant amount of isolated antibody is incubated with a fixed... 

FIGURE 23-3 Radioimmunoassay (RIA). (a) A low concentration of radiolabeled hormone (red) is incubated with (T) a fixed amount of antibody specific for that hormone or (2) a fixed amount of antibody and various concentrations of unlabeled hormone (blue). In the latter case, unlabeled hormone competes with labeled hormone for binding to the antibody the amount of labeled hormone bound varies inversely with the concentration of unlabeled hormone present, (b) A radioimmunoassay for adrenocorticotropic hormone (ACTH). A standard curve of the ratio [bound] to [unbound radiolabeled ACTH] vs. [unlabeled ACTH added] is constructed and used to determine the amount of (unlabeled) ACTH in an unknown sample. If an aliquot containing an unknown quantity of unlabeled hormone gives, say, a value of 0.4 for the ratio [bound]/[unbound] (see arrow), the aliquot must contain about 20 pg of ACTH. 

Radioimmunoassay (RIA) and ELISA are two very sensitive techniques for detecting and quantifying hormones. 

Rosalyn S. Yalow, Roger C. L. Guillemin, and Andrew V. Schally Physiology/Medicine Radioimmunoassay thyrotropin-releasing hormone... 

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