High with fluorescence detection HPLC

Alkylphenol derivatives in the Mediterranean Sea The behaviour of lipophilic metabolites of AgPEO in the Venice lagoon, Italy, was studied in detail by Marcomini et al. [2,3]. Salinity in this estuary is in the range of 25-35%o, and both industrial and domestic wastewater is discharged into the lagoon. Water samples were analysed by high performance liquid chromatography with fluorescence detection (HPLC-FL) [2]. 

In the mid 1980s, Krahn and co-workers (1984) developed a moderately inexpensive, rapid screening method for estimating the relative amount of PAH metabolites present in fish bile. Their method used high-performance liquid chromatography with fluorescence detection (HPLC-F) for estimating bile metabolites. In brief, fluorescent... 

Very recently, HPLC with fluorescence detection was recommended for improving detection sensitivities of betalains. " While this technique may be worthwhile for betaxanthin analyses, its use for betacyanins cannot be recommended. Although this technique represents a worthwhile approach requiring low amounts of solvent and sample and generally characterized by a high separation efhciency, only one study dealt with the use of capillary zone electrophoresis for betalain analyses. ... 

You have the task of purchasing some n-hexane for use in three different applications (i) pesticide analysis by gas chromatography, (ii) as a solvent to extract some non-polar high-boiling (200-300°C) oils from a soil sample, and (iii) as a mobile phase for HPLC analysis with UV detection. List and contrast the performance characteristics you need to take into account for purchasing the appropriate grade of hexane in each case. n-Hexane boils at about 70°C. Will any of your choices of hexane be suitable for use for HPLC analysis with fluorescence detection Explain your decision. 

In this regard several sophisticated chromatographic methods, with a quantification limit down to about 0.2 ng/g, have been developed and published for the determination of zearalenone. The methods were mainly based on high-performance liquid chromatography (HPLC) with fluorescence detection (Krska 1998 Visconti and Pascale 1998 Schuhmacher et al. 1998 Tanaka et al. 2000), but HPLC with mass spectrometry detection was also used (Shirai et al. 2000 Josephs et al. 2001). 

HPLC methods with fluorescence detection have also been developed for the determination of nitro-policyclic aromatic hydrocarbons (PAHs) (among which 9-nitroanthracene and 1-nitronaph-thalene) [238] in atmosphere. Samples have been collected in a standard high-volume sampler with a Teflon-coated glass fiber filter, and the Soxhlet extraction was performed with dichloromethane as the solvent. RP HPLC/UV techniques are used for the determination of aldehydes, ketones, and carbonylic compounds after derivatization with DNPH [239],... 

Nowadays, the preferred methodology is the separation and quantification of porphyrins by high-performance liquid chromatography (HPLC) with fluorescence detection. Thus, a method that detects the main isomers of specific porphyrins is recommended, as a high number of different isomers exist, especially in pathological conditions, and the relative abundance of the different isomers supports the distinction of the different porphyrias (Table 7.3.1). 

A large number of analytical methods for detection of CWA degradation products are available. Gas chromatography/mass spectrometry (GC/MS) and high performance liquid chromatography (HPLC) with fluorescence detection are the most widely used.6,7 However, limitations of these methods include complicated derivatization procedures, long analysis time, and difficulty in miniaturization. 

High-pressure liquid chromatography (HPLC) with fluorescence detection, ion-trap mass spectrometry (ITMS), and capillary electrophoresis (CE) with ultraviolet detection, three novel detection techniques for the analysis of cocaine in hair, have been evaluated by Tagliaro et aU°- and Traldi et al. The HPLC technique was highly sensitive, capable of detecting 0.015 ng/mg of cocaine in hair. The CE method was sensitive and highly selective. ITMS analysis of hair readily demonstrated the presence of cocaine in hair, but cocaine metabolites were more difficult to identify. 

Modern tHcy methods include enzyme immunoassays and chromatographic-based methods. In practice, immunoassays " are most often used for routine purposes (e.g., fluorescence polarization immunoassay IFPIA] as run on Abbott s IMx and AxSYM platforms) Chromatographic assays include amino acid analysis high-performance liquid chromatography (HPLC) with ultraviolet, fluorescence, or electrochemical detection ° " capillary electrophoresis with fluorescence detection gas chromatography-mass spectrometry (GC-MS) and liquid chromatography with tandem MS (MS-MS). 

In an analysis of B[a]P and coal tar pitch volatiles in workplace air conducted by Ny et al. (1993), urine samples were analyzed for the pyrene metabolite 1-hydroxypyrene, and a high correlation between levels of this biomarker and PAH air levels was observed. Analyses were also conducted by HPLC with fluorescence detection. Tolos et al. (1990) reported results of 1-hydroxypyrene urinalysis for aluminum reduction plant workers, and showed a strong positive correlation between the compound and 17 environmental PAHs. This work verified the choice by earlier researchers (Jongeneelen et al. 1988) of the pyrene metabolite as a useful marker of exposure to PAHs. Elovaara et al. (1995) also demonstrated the usefulness of 1-hydroxypyrene as a biomarker for exposure to naphthalene and 10 other PAHs for creosote impregnation plant workers. Particulate PAHs were Soxhlet extracted with cyclohexane and analyzed by HPLC with fluorescence detection. 

HPLC-FD High-performance liquid chromatography combined with fluorescence detection has been used for the first analyses of nucleobases in carbonaceous chondrites (/5). More recently, pre-column derivatization of amino acid with o-phthaldialdehyde/N-acetyl-L-cysteine (OPA/NAC) has allowed the detection and enantiomeric separation of a few pmol of amino acids 16),... 

Heybroek, W.M. Caulfield, M. Johnston, A. Himer, P. Automatic on-line extraction coupled with electrochemical detection as an improved method for the HPLC co-analysis of codeine and morphine in plasma and gastric juice. J.Pharm.Biomed.Anal., 1990, 8, 1021-1027 Chen, Z.R. Bochner, F. Somog3d, A. Simultaneous determination of codeine, norcodeine and morphine in biological fluids by high-performance hquid chromatography with fluorescence detection. J.Chromatogr., 1989, 491, 367-378... 

Peyton, A.L. Carpenter, R. Rutkowski, K. The stereospecific determination of fluoxetine and norfluoxetine enantiomers in human plasma by high-pressure liquid chromatography (HPLC) with fluorescence detection. Pharm.Res., 1991, 8, 1528-1532... 

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