Multiple simultaneous synthesis

Meyers HV, Dilley GJ, Durgin TL, Powers TS, Winssinger NA, Zhu H, Pavia MR. Multiple simultaneous synthesis of phenolic libraries. Mol Diversity 1995 1 13-20. 

Cellulose supports in the form of paper sheets have been used for the multiple simultaneous synthesis of peptides. An amino acid is attached to the surface of the cellulose support through the formation of an ester bond at the C-terminal end of the amino acid [38,39], Typical loading levels for this support are in the range of 0.5-0.6 pmol/cm2. Peptide libraries synthesized on this support have been primarily tested while still attached to the cellulose surface. Cotton has also been used as a support for the synthesis of peptide libraries exhibiting loading levels ofaround 0.04-0.12 mmol/g (1-3 pmol/cm2) [40],... 

While gellike and macroporous resins cover the vast majority of SPS, the use of other supports has also been explored. Cellulose (33, 34) in the form of paper sheets has been employed for multiple simultaneous SPS of peptides with a relatively low loading of 0.5-0.6 j,mol/cm. Cotton (35) has been used for the same application with a loading of around 0.1 mmol/g. Glass (36) was among the first supports used for the synthesis of large numbers of peptides, due to its chemical inertness and solidity. Various polymeric membranes (37, 38) were also used to prepare peptides on SP. Several of these supports will be mentioned also as related to combinatorial hbrary synthesis (see Section 6.4.1 and 6.4.2). 

Houghten, R. A., DeGraw, S. T., Bray, M. K., Hoffmann, S. R., and Frizzell, N. D. (1986) Simultaneous multiple peptide synthesis The rapid preparation of large numbers of discrete peptides for biological, immunological, and methodological studies. BioTechniques 4, 522-528. 

Frank, R. and Coring, R. (1988) Simultaneous multiple peptide synthesis under continuous flow conditions on cellulose paper discs as segmental solid supports. 

Schnorrenberg, G. and Gerhardt, H. (1989) Fully automatic simultaneous multiple peptide synthesis in micromolar scale Rapid synthesis of series of peptides for screening in biological assays. Tetrahedron 45, 7759-7764. 

RA Houghten, ST DeGraw, MK Bray, SR Hoffmann, ND Frizzell. Simultaneous multiple peptide synthesis. Biotechniques 4 522-528, 1986. 

R Frank. Strategies and techniques in simultaneous multiple peptide synthesis based on the segmentation of membrane type supports. Bioorg Med Chem Lett 3 425-430, 1993. 

Multiple peptide synthesis has also been achieved by using polystyrene-grafted polyethylene films (PS-PE) which are produced using X-ray irradiation.22 Small areas of the film (typically 1.5 x 3 cm) serve as reaction compartments where coupling can be carried out separately while washing and deblocking is carried out simultaneously. 

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