High-speed countercurrent chromatograph

Kapadia, G.J., Oguntimein, B., and Shukla, Y.N., High-speed countercurrent chromatographic separation of biflavonoids from Garcinia kola seeds, J. Chromatogr. A, 673, 142, 1994. 

Name and acronym Centrifugal partition chromatograph (CPC) High-speed countercurrent chromatograph (HSGGG)... 

Source From Improved high-speed countercurrent chromatograph with three multilayer coils connected in series, n. Separation of various biological samples with a semipreparative column in J. Chromatogr. ... 

Kitazume, E. High-speed countercurrent chromatograph device and analysis method. Jpn. Kokai Tokkyo Koho JP. 2005,315731 A. 

Vanhaelen-Fastre, R. Diallo, B. Jaziri, M. Faes, M.L. Homes, J. Vanhaelen, M. High-speed countercurrent chromatographic separation of taxol and related diterpenoids from Taxus baccata. J. Liq. Chromatogr. 1992,15 (4), 697-706. 

High speed countercurrent chromatography (HSCCC) produces highly efficient chromatographic separations of solutes without the use of solid supports Thus the method eliminates all complications caused by the solid support, such as adsorptive loss and deactivation of samples, tailing of solute peaks, contamination, etc. As with other CCC schemes, HSCCC utilizes two immiscible solvent phases, one as a stationary phase and the other as a mobile phase, and the separation is highly dependent on the partition coefficient values of the solutes, i.e., the ratio of the solute concentration between the mobile and stationary phases. Therefore the successful separation necessitates a careful search for the suitable two-phase solvent system that provides an ideal range of the partition coefficient values for the applied sample. 

High-speed countercurrent chromatography (HSCCC) and centrifugal partition chromatography (CPC) are two very promising chromatographic techniques which have been successfully applied to the purification of natural products. 

High-speed countercurrent chromatography (HSCCC) is a relatively new technique. It is a liquid-liquid partitioning chromatographic method in which the stationary phase is immobilized by a centrifugal force. HSCCC is the most advanced form of CCC in terms of partition efficiency and separation time. As solvent system, a mixture in different ratio is usually used, depending on the flavonoids nature ethyl acetate/... 

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