High-resolution spectrometry, drug

Mass spectrometry has become one of the most important tools for analyzing proteins in complex biological samples. The ability to separate proteins and peptides in high resolution has made possible the simultaneous identification of hundreds of proteins within samples (for reviews, see Gingras et al., 2005 Hamdan and Righetti, 2005 Siuzdak, 2006). Proteins can be analyzed for their presence or compared between samples for their relative expression level. One cell population treated with a drug candidate, for instance, can be compared by mass spec to another sample as control to assess the affect of the drug on expression levels of certain proteins. 

Thomas, S.R. and Gerhard, U. (2004) Open-access high-resolution mass spectrometry in early drug discovery. Journal of Mass Spectrometry, 39, 942-948. 

Detection and characterization of metabolites in biological matrices using mass defect filtering of liquid chromatography/high resolution mass spectrometry data. Drug Metab. Dispos. 2006, 34, 1722. 

Mortishire-Smith, R. J., O Connor, D., Castro-Perez, J. M., and Kirby, J. (2005). Accelerated throughput metabolic route screening in early drug discovery using high-resolution liquid chromatography/quadrupole time-of-flight mass spectrometry and automated data analysis. Rapid Commun. Mass Spectrom. 19 2659-2670. 

Evans, E. H. Wolff, J.-C. Eckers, C. 2001. Sulfur-specific detection of impurities in cimetidine drug substance using liquid chromatography coupled to high resolution inductively coupled plasma mass spectrometry and electrospray mass spectrometry. Anal. Chem., 73,4722-4728. 

G.A. MacKay and G. D. Reed, Application of capillary supercritical fluid chromatography, packed column supercritical fluid chromatography, and capillary supercritical fluid chromatography-mass spectrometry in the analysis of controlled drugs, J. High Resolut. Chromatogr., 14 531 (1991). 

The chromatographic separation of enantiomers as diastereomers was first developed using GLC (1). Subsequently, many separations using GLC were reported, but modern LC has dominated the field of enantiospedfic drug analysis in recent years. Nevertheless, the arrival of high-resolution capUlary GLC has revived interest in the use of indirect enantiomer separation via this type of chromatography, and today GLC remains important in the analytical separation of enantiomers after derivatization with CDAs. The availability of sensitive detection methods, for example, mass spectrometry, electron capture, etc., enhances the applicability of GLC in indirect enantiospedfic drug analysis. 

Tomlinson, A.J. Benson, L.M. Naylor, S. Nonaqueous Solvents in the online Capillary Electrophoresis Mass Spectrometry Analysis of Drug Metabolites. HRC - Journal of High Resolution Chromatography, 17, 175-177 (1994). 

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