High-performance liquid chromatography separation, preparative

Polystyrene resins having pendant nucleic acid bases were prepared. They were found to be suitable high performance liquid chromatography separating effectively N-sub-stituted nucleic acid bases. Retention times and peak resolution values indicate that the specific base-base interaction between the solute and the resin plays an important role in the separation process82. ... 

Kim K, Kim D (2005) High-performance liquid chromatography separation characteristics of molecular-imprinted poly(methacrylic acid) microparticles prepared by suspension polymerization. J Appl Polym Sci 96(1 ) 200—212... 

Saleh, F. Y., Ong, W. A., and Chang, D. Y., Structural features of aquatic fulvic acids analytical and preparative reversed phase high performance liquid chromatography separation with photodiode array detection. Anal. Chem., 61, 2792-2800, 1989. 

Despite the difficulties caused by the rapidly expanding literature, the use of chiral stationary phases (CSPs) as the method of choice for analysis or preparation of enantiomers is today well established and has become almost routine. It results from the development of chiral chromatographic methods that more than 1000 chiral stationary phases exemplified by several thousands of enantiomer separations have been described for high-performance liquid chromatography (HPLC). 

High performance liquid chromatography (HPLC) has been by far the most important method for separating chlorophylls. Open column chromatography and thin layer chromatography are still used for clean-up procedures to isolate and separate carotenoids and other lipids from chlorophylls and for preparative applications, but both are losing importance for analytical purposes due to their low resolution and have been replaced by more effective techniques like solid phase, supercritical fluid extraction and counter current chromatography. The whole analysis should be as brief as possible, since each additional step is a potential source of epimers and allomers. 

Snyder, L. R., Dolan, J. W., and Cox, G. B., Preparative high-performance liquid chromatography under gradient conditions. III. Craig simulations for heavily overloaded separations, J. Chromatogr., 484, 437, 1989. 

Lee, A. L., Liao, A. W., and Horvath, Cs., Tandem separation schemes for preparative high-performance liquid chromatography of proteins,. Chromatogr., 443, 31, 1988. 

Siitfeld, R., Preparative liquid chromatography with analytical separation quality interval injection/displacement reversed-phase high-performance liquid chromatography, ]. Chromatogr., 464, 103, 1989. 

Threadgill, G.J., Conrad, R.C., Cannon, M., Craven, G.R. (1987). A rapid and preparative method for the separation of yeast ribosomal proteins by using high-performance liquid chromatography. Biochem. J. 244, 523-532. 

In the separation of biomolecules, sample preparation almost always involves the use of one or more pretreatment techniques. With high-performance liquid chromatography (HPLC), no one sample preparation technique can be appHed to all biological samples. Several techiques may be used to prepare the sample for injection. For example, complex samples require some form of preffactionation before analysis, samples that are too dilute for detection require concentration before analysis, samples in an inappropriate or incompatible solvent require buffer exchange before analysis, and samples that contain particulates require filtration before injection into the analytical instrument. 

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