High performance liquid chromatography qualitative applications

In earlier times, thin-layer chromatography (TLC), polyamide chromatography, and paper electrophoresis were the major separation techniques for phenolics. Of these methods, TLC is still the workhorse of flavonoid analysis. It is used as a rapid, simple, and versatile method for following polyphenolics in plant extracts and in fractionation work. However, the majority of published work now refers to qualitative and quantitative applications of high-performance liquid chromatography (HPLC) for analysis. Llavonoids can be separated. 

J.O. Doali A.A. Juhasz, Application of High-Performance Liquid Chromatography to the Qualitative Analysis of Compounds of Propellant and Explosives Interest , JChromatogrSci 12 (1), 51 -56 (1974) 92) F.T. Sweeney P.W.D. 

Before embarking on a study of high-performance liquid chromatography (HPLC), it is instructive to take a look at how this analytical technique emerged and to consider its relative importance in the field of analytical chemistry. Although HPLC is used to determine what is in a sample (qualitative analysis), its primary application is as a quantitative analytical tool and as such it plays a key role in analytical methods, since the analytical technique is only one part of the overall analytical method. An analytical method may be thought of as consisting of five distinct parts ... 

Analysis is an integral part of research, clinical, and industrial laboratory methodology. The determination of the components of a substance or the sample in question can be qualitative, quantitative, or both. Techniques that are available to the analyst for such determinations are abundant. In absorption spectroscopy, the molecular absorption properties of the analyte are measured with laboratory instruments that function as detectors. Those that provide absorbance readings over the ultraviolet-visible (UV-vis) light spectrum are commonly used in high-performance liquid chromatography (HPLC). The above method is sufficiently sensitive for quantitative analysis and it has a broader application than other modes of detection. 

Thin layer chromatography (TLC) is used in the pharmaceutical industry as a tool for quantitative and qualitative analysis of steroids the technique is useful for solving difficult analytical problems even in small laboratories equipped only with a basic setup. Although the use of high performance liquid chromatography (HPIX ) has increased rapidly in this field, and HPLC has superseded TLC for several applications, because of the simplicity and resolving power of TLC, it is sufficient to solve many analytical problems in steroid analysis. In the last decade many aspects of steroid analysis have been covered in a number of books, chapters, reviews and fundamental papers (1-8) these publications should be regarded as the basic source of informatitHi on this subject. 

A third area of development in carbohydrate l.c. analyses is in the combined techniques (see Section IV,3) and other methods that provide qualitative, as well as quantitative, information about sample constituents, such as high-performance liquid affinity chromatography. The use of specific lectin- and monoclonal antibody-based, stationary phases for analytical and preparative applications is now being considered. The basic concepts of these techniques have been reviewed - and their applications to carbohydrates have been discussed. 

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