High-performance liquid chromatography glycolipids

Lampio, A. and Finne, J., Sugar analysis of glycoproteins and glycolipids after methanolysis by high-performance liquid chromatography with pulsed amperometric detection, Anal. Biochem., 197, 132, 1991. 

Preparative and Analytical High Performance Liquid Chromatography of Glycolipids... 

Suzuki, A. Handa, S, Yamakawa, Y. Separation of molecular species of higher glycolipids by high performance liquid chromatography of their 0-acetyl-N-p-nitrobenzoyl derivatives. 

In this manuscript, we will first describe the newly developed high performance liquid chromatography of cerebroside, sulfatide, and other minor galactolipids. This method allows complete analysis of a very small amount of these glycolipids in cell or membrane preparations. This will be followed by a description of our new method of determining surface galactolipids and its application to myelin cerebrosides. 

The following chapters in Advances in Lipid Methodology, Volume 3 (Cl) Chapter 3, Separation of phospholipid classes by high-performance liquid chromatography. W. W. Christie (C2) Chapter 6, Plant glycolipids Structure, isolation and analysis, E. Heinz. 

Sugawara, T and T. Miyazawa. Separation and detection of glycolipids from edible plant sources by high-performance liquid chromatography and evaporative light scattering detection. Lipids 1999,34 1231-1237. 

Snada, S., Uchida, Y., Anraku, Y, Izawa, A., Iwamori, M., and Nagai, Y., Analysis of ceramide and monohexaosyl glycolipid derivatives by high-performance liquid chromatography and its application to the determination of the molecular species in tissues, J. Chromatogr., 400, 223-231, 1987. 

Analytical methods for phospholipid and glycolipid classes by normal-phase high-performance liquid chromatography (HPLC) have been incompletely reviewed. Silica columns with evaporative light scattering detection (ELSD) were mostly used. The poly(vinyl alcohol)-grafted silica column with ELSD could be used to separate them, simultaneously. 

Smith, LA. Norman, HA. Cho, S.H. Thompson, GA. Jr. Isolation and quantitative analysis of phosphatidylglycerol and glycolipid molecular species using reversed-phase high-performance liquid chromatography with flame ionization detection, J.Chromatogr., 1985,346, 291-299. 

Hara, S., Takemori, Y., Yamaguchi, M., Nakamura, M., and Ohkura, Y., 1987, Fluorimetric high-performance liquid chromatography of N-acetyl- and N-glycolylneuraminic acids and its application to their microdetermination in human and animal sera, glycoproteins, and glycolipids. Anal. Biochem. 164 138-145. 

Some preliminary fractionation of lipid samples into simple lipid, glycolipid and phospholipid groups may then be desirable to facilitate their analysis or the isolation of single lipid classes on a small scale. The last objective can be accomplished by high-performance liquid, thin-layer or ion-exchange chromatography. 

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