High performance liquid chromatography chiral recognition mechanism

In another study, the authors reported a comparative study of the enantiomeric resolution of miconazole and the other two chiral drugs by high performance liquid chromatography on various cellulose chiral columns in the normal phase mode [79], The chiral resolution of the three drugs on the columns containing different cellulose derivatives namely Chiralcel OD, OJ, OB, OK, OC, and OE in normal phase mode was described. The mobile phase used was hexane-isopropanol-diethylamine (425 74 1). The flow rates of the mobile phase used were 0.5, 1, and 1.5 mL/min. The values of the separation factor (a) of the resolved enantiomers of econazole, miconazole, and sulconazole on chiral phases were ranged from 1.07 to 2.5 while the values of resolution factors (Rs) varied from 0.17 to 3.9. The chiral recognition mechanisms between the analytes and the chiral selectors are discussed. [Pg.52]

Anionic Catalysis Several bulky methacrylates afford highly isotactic, optically active polymers having a single-handed helical structure by asymmetric polymerization. The effective polymerization mechanism is mainly anionic but free-radical catalysis can also lead to helix-sense-selective polymerization. The anionic initiator systems can also be applied for the polymerization of bulky acrylates and acrylamides. The one-handed helical polymethacrylates show an excellent chiral recognition ability when used as a chiral stationary phase for high-performance liquid chromatography (HPLC) [97,98]. [Pg.769]

Hui, F. (2004) High Performance Liquid Chromatography and Capillary Electrophoresis Chiral Recognition Mechanisms Using Glycopeptide Macrocyclic Antibiotics as Selectors, Fenxi Huaxue 32, 964-968. [Pg.363]