High-performance gel permeation chromatography

High-Performance Gel Permeation Chromatography Characterization of Oligomers Used in Coatings Systems... 

Barth, H. G. and Regnier, F. E., High-performance gel permeation chromatography of water-soluble cellulosics, /. Chromatogr., 192, 275, 1980. 

Barth, H.G. and Regnier, F.E. "High Performance Gel Permeation Chromatography of Water-Soluble Cellulosics." J. Chromatog.(19801 192, 275 293. 

Use in High-Performance Gel Permeation Chromatography of PoIy(ethyIene terephthalate)... 

A continuous capillary viscosity detector has been developed for use in High Performance Gel Permeation Chromatography (HPGPC). This detector has been used in conjunction with a concentration detector (DRI) to provide information on the absolute molecular weight, Mark-Houwink parameters and bulk intrinsic viscosity of polymers down to a molecular weight of about 4000. The detector was tested and used with a Waters Associates Model 150 C ALC/GPC. The combined GPC/Viscometer instrumentation was automated by means of a micro/mini-computer system which permits data acquisition/reduction for each analysis. 

Figure 6. High performance gel permeation chromatography (HPGPC) profiles of MHR treated with technical enzyme preparations (30°C, 3 hours). HPGPC conditions BioGel TSK 40xl, 30xl, 20xl in series, eluted with 0.4 M NaAc, pH 3.4, at 30°C.

HPLC with silica columns has been used to separate PCBs from toxaphene [138-140] as well as for isolating toxaphene components [65, 92, 94]. Marth et al. found that HPLC silica phases with a large surface (here 650 m2/g) were suitable for a PCB/toxaphene group separation but not those with a small one (here 170 m2/g) [138]. The elution order of toxaphene standards by RP-HPLC on octadecylsilane phases was also determined. However, as for amino-, cyano-, and nitro-modified HPLC phases [138], no complete separation of PCBs from toxaphene could be achieved [94,141]. Brumley et al. suggested the use of high performance gel-permeation chromatography for the PCB/toxaphene group separation [142],... 

The molecular weight distribution of povidone can best be determined with the aid of high-performance gel permeation chromatography. Figure r9 gives a qualitative comparison between povidone K 17 and povidone K 30 in a gel permeation chromatogram marked at a molecular weight of 35 000. 

HP-SEC, also called high-performance gel-permeation chromatography (HP-GPC), is performed on porous stationary phases and separates analytes according to their molecular mass or their hydrodynamic volume. As a nonretentive separation mode, HP-SEC is usually operated with isocratic elution using aqueous low salt mobile... 

Brumley, W.C., C.M. Brownrigg, and A.H. Grange. 1993. Determination of toxaphene in soil by electron-capture negative ion mass spectrometry after fractionation by high-performance gel permeation chromatography. J. Chromatogr. 633 177-183. 

ASE, accelerated solvent extraction HPGPC, high-performance gel permeation chromatography OSUA, oligomers of sodium undecylenic acid SEE, supercritical fluid extraction SDpCD, sulfobutyl ether p-cyclodextrin MpCD, methyl p-cyclodextrin PFOS , perfluorooctanic sulfate DMSO, dimethyl sulfoxide THA+, tetrahexylammonium SPME, solid-phase microextraction STDC, sodium taurodeoxycholate LIE, laser-induced fluorescence LLE, liquid-liquid extraction. 

M. Terbojevich, A. Cosani, B. Focher, E. Marsano, High-performance gel-permeation chromatography of chitosan samples, Carbohydr. Res. 250 (1993) 301-314. 

The key step in the synthesis of 168 and 169 is an oxidative acetylenic coupling reaction of open-top precursor cavitands 170 and 171. Intramolecular coupling of 171 affords the basket 169 along with a dimeric structure and the switchable tube 172, in a ratio of 2/6 10 1.The two products can be separated by preparative high-performance gel-permeation chromatography (2007ACE260). 

J. V. Dawkins, G. Yeadon, High Performance Gel Permeation Chromatography in Development in Polymer Characterization vol 1, Chapter 23, Applied Science Publishers Ltd., London, 1978. 

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