Hepatic lectins

Incomplete (N-linked) glycosylation prompts decreased in vivo activity due to more rapid hepatic clearance of the EPO molecule. Enzymatic removal of terminal sialic acid sugar residues from oligosaccharides exposes otherwise hidden galactose residues. These residues are then free to bind specific hepatic lectins, which promote EPO removal from the plasma. The reported plasma tm value for native EPO is 4-6 h. The tm for desialated EPO is 2 min. Comparison of native human EPO with its recombinant form produced in CHO cells reveals very similar glycosylation patterns. 

Lehrman MA, Pizzo SV, Imber Ml, Hill RL. The binding of fucose-containing glycoproteins by hepatic lectins. Re-examination of the clearance from blood and the binding to membrane receptors and pure lectins. J. Biol. Chem. 1986 261 7412-7418. 

The canonical asialoglycoprotein receptor (ASGPR) is the hepatic lectin (HL) common to mammals, first reported in the rat in 1974 by Ashwell and co-workers [98] (reviewed in [99,100,101]. Rat HL is a complex of two homologous C-type lectins (46kDa HL-1 and... 

The mammalian hepatic lectin (also referred to as the asialoglycoprotein receptor) is found on the surface of liver cells. The protein directs the clearance from the serum... 

B. Low Molecular Weight Trimeric Galactose Displays Can Be Potent Ligands for the Hepatic Lectin on the Cell Surface... 

C. Low Molecular Weight Trimeric Galactose Arrays More Effectively Inhibit Cell-Surface-Bound than Soluble Hepatic Lectin... 

D. Ligand Spacing Allows Selective Targeting of the Hepatic Lectin over a Related Receptor... 

E. Applications of Ligands for the Hepatic Lectin Exploiting Hepatic Lectin for Selective Targeting... 

The hepatic lectin is a key model system for the development of receptor-mediated drug delivery [125-131]. The observations that the hepatic lectin can endocytose... 

Using Synthetic Saccharide Determinants for Gene Delivery via Hepatic Lectin... 

Selective Uptake by the Hepatic Lectin of an Antisense Oligonucleotide-Saccharide Epitope Conjugate... 

In analogy to the results obtained for targeting genes, it is expected that the endo-cytotic abilities of the hepatic lectin could also delivery oligonucleotides for antisense... 

Cholesterol-Lowering Conjugates Designed to Function Through the Hepatic Lectin... 

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