Heat shock temperatures, effect

Currie, R.W. (1987). Effects of ischemia and perfusion temperature on the synthesis of stress-induced (heat shock) proteins in isolated and perfused rat hearts. J. Mol. Cell. Cardiol. 19, 795-808. 

Plesofsky-vig N, Brambl R (1985) The heat shock response of fungi. Exp Mycol 9 187-194 Ploeg AT, Stapleton JJ (2001) Glasshouse studies on the effects of time, temperature and amendment of soil with broccoli plant residues on the infestation of melon plant by Meloidogyne incognita and M. javanica. Nematology 3 855-861 Pokharel RR (1995) Effect of crop rotation and solarization on the population densities of rice root nematode Hirschmanniella spp. in Nepal. Int Rice Res Notes 20 28-29... 

Figure 7.11. The effects of exposure temperature on protein synthetic patterns of isolated gill tissue from specimens of 13°C-acclimated Tegula funebralis. Autoradiographic images illustrate newly synthesized (35S-labeled) proteins of several size classes (molecular mass standards are shown in the left lane). Two specimens from each temperature of incubation are shown. At temperatures above 24°C, synthesis of heat-shock proteins in the molecular mass ranges of 38, 70, 77, and 90 kDa is induced. Hsp synthesis becomes an increasingly large fraction of protein synthesis as exposure temperature increases, and by 38°C, only synthesis of hsp70 is observed. By 39° C, no protein synthesis takes place. (Figure modified after Tomanek and Somero, 1999.)...

Fish are ectothermic vertebrates, and as such are susceptible to fluctuations in temperature of their immediate environment. This has relevance to sewage and industrial effluents, which can be significant sources of thermal pollution in aquatic environments. Recent evidence demonstrating that heat shock can induce apoptosis in a variety of fish tissues21,111,136 suggests that some deleterious effects of thermal pollution may be mechanistically linked to increased apoptosis of susceptible cell types. 

Brassinosteroids (BSs) represent a new group of plant hormones that possess a broad spectrum of physiological activities (1,2). A most Intriguing property of BSs Is their capacity to Increase stress resistance In plants, but the mechanism of such an antistress activity still remains unknown (1). As cell stress resistance Is usually associated with stress protein synthesis (3 4) our aim was to study the BS effect on protein synthesis and ultrastructure of wheat leaf cells at normal temperature and under heat shock conditions. We have also studied the Influence of BSs on mesophyll cell ultrastructure under saline stress. 

The assay is especially applicable to experiments with conditional transport mutants where the efficiency of NLS-directed transport is best measured soon after the cells are transferred to nonpermissive conditions. In practice, the relative rate of NLS-GFP import in temperature-sensitive strains can be measured immediately on transfer of cells to nonpermissive temperatures (Shulga et al., 1996). This facility minimizes pleiotropic or secondary effects that commonly develop in cells held at nonpermissive conditions for extended periods. As described in the next section, some transport defects are too subtle to detect using methods that depend on the steady-state mislocalization of a reporter protein. The NLS-GFP fusion protein has been used to show that NLS-GFP reimport is normal in cells that had already exhibited a defect in mRNA export 10 min after transfer to a nonpermissive temperature (Murphy and Wente, 1996). Also, Saavendra et al. (1996) showed that in heat-shocked cells the export of SSA4 and SSAl mRNAs (which encode Hsp70s) occurs efficiently, whereas poly(A) RNA export and NLS-GFP import were significantly impaired. 

Perhaps the most common types of electrical equipment found in a laboratory are the devices used to supply the heat needed to effect a reaction or a separation. These include ovens, hot plates, heating mantles and tapes, oil baths, salt baths, sand baths, air baths, hot-tube furnaces, hot-air guns, and microwave ovens. The use of steam-heated devices rather than electrically heated devices is generally preferred whenever temperatures of 100 °C or less are required. Because they do not present shock or spark risks, they can be left unattended with assurance that their temperature will never exceed 100 °C. 

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