Haptenes, dextrans

Halohydrocarbons, as solvents for acetylation of cellulose, I, 313 Haptenes, dextrans as, II, 215 pneumococcus polysaccharide as, II, 221... 

It is possible for a severe dextran-induced anaphylactic/ anaphylactoid reaction to develop despite prophylaxis with monovalent hapten dextran (30). This has been reported in a 60-year-old patient with multiple trauma, who received a dextran infusion for prophylaxis of thrombosis due to severe thrombocjdosis in the late postoperative period. The causal relation to dextran was considered likely, although no serum sample was taken before the reaction, due to the close time relation to the infusion of dextran 60. In addition, there were high titers of dex-tran-reactive antibodies in the blood drawn immediately after the reaction occurred. 

Bocher, M., Giersch, T., and Schmid, R.D. (1992) Dextran, a hapten carrier in immunoassays for s-triazines. A comparison with ELISAs based on hapten-protein conjugates./. Immunol. Meth. 151, 1-8. 

In 1996, Gauglitz and coworkers coated surfaces with various amino-and carboxy-substituted polymers [198], The polymers tested were branched poly-(ethyleneimine), a,co-amino-functionalized PEG, chitosan, poly(acrylamide-co-acrylic acid) and an amino-modified dextran. The amino-substituted polymers were immobilized on glass by first immobilizing an aminosilane, followed by succinic anhydride/A-hydroxysuccinimide linker chemistry. Poly(acrylamide-co-acrylic acid) was directly coupled to an aminosilanized surface. When probed with 1 mg mL 1 ovalbumin solution, nonspecific adsorption was lowest for the dextran derivative. Notably, nonspecific adsorption increased in most cases when a hydrophobic hapten (atrazine) was coupled to the polymer-modified surface. 

Quantitative hapten inhibition tests were also performed.73 The amount of myeloma protein-dextran complex that formed in individual tests was measured by the determination of protein in the precipitate, using a colorimetric method.74 The precipitin tests were performed in a final volume of 0.2 mL, which comprised 80 pL of 0.02 M phosphate buffer of pH 7, 20 pL of ascitic fluid or purified myeloma protein, and 100 pL of dextran B-1355S solution in phosphate buffer of pH 7. The extent to which glucose... 

Ljungstrom KG. Safety of dextran in relation to other colloids—ten years experience with hapten inhibition. Infusionsther Transfusionsmed 1993 20(5) 206-10. 

Ljungstrom KG, Wilknan B, Hedin H. Hapten inhibition of dextran anaphylaxis. Nine years of post-marketing surveillance of dextran 1. Ann Fr Anesth Reanim 1993 12(2) 219-22. 

Allhoff T, Lenhart FP. Schwere dextraninduzierte anaphylactische/anaphylactoide Reaktion (DIAR) trotz Haptenprophylaxe. [Severe dextran-induced anaphylactic/ana-phylactoid reaction despite preventive hapten administration.] Infusionsther Transfusionsmed 1993 20(6) 301-6. 

Berg EM, Fasting S, SeUevold OF. Serious complications with dextran-70 despite hapten prophylaxis. Is it best avoided prior to delivery Anaesthesia 1991 46(12) 1033-5. 

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