Haemoglobin derivatives

Haemoglobin-derived haem iron has multiple pro-inflammatory effects resulting from its ability to initiate decomposition of lipid hydroperoxides from PUFAs. In... 

Bennett, J.E. and Ingram, D.J.E. 1956. Analysis of crystalline haemoglobin derivatives by paramagnetic resonance. Nature 177 275-276. 

No examples for quantification in the product ion scan mode were found in the literature even though data processing would allow extraction of selected ions, integration of related signal areas, and summation for quantification. This procedure has been used by John et al. for the determination of the human haemoglobin derived peptide hHEM-y 130-146 in plasma [102], However, quantification especially of small molecule analytes is best performed in the MRM mode that is addressed below. 

Iron compounds are particularly well-suited for studies of the Moss-bauer Effect (80) and a number of haemoglobin derivatives have been investigated by means of this technique (81, 5). Mossbauer spectra should, in principle, yield information about spin-states, but the results as yet are not very conclusive (82, 10). Better results are being obtained with the application of a magnetic field (82). 

The reflectance of the dye that has formed, is measured at 535 nm during the incubation and measurement phase for a period of 45 to 180 seconds - depending upon the time until a stable reflectance value has developed (this is due to the presence of various haemoglobin derivatives). The haemoglobin concentration can be calculated by means of the calibration data obtained by a 2-point calibration (high/low). 

The reactions of haemoglobin derivatives in their oxidised (Fe ") form with ligands have also been studied kinetically. The reactions tend to be... 

A large number of haemoglobin derivatives are known, some of which occur naturally. The following are among the most important. 

Naturally occurring free radicals in phthalocyanine, chlorophyll and haemoglobin derivatives Naturally occurring free radicals in phthalocyanine and other biological substances... 

Henry Y, Banerjee R. 1973. Electron paramagnetic studies of nitric oxide haemoglobin derivatives isolated subunits and nitric oxide hybrids. JMo/5/o/73(4) 469—482. 

Stotter, 1979) in which single pendant arms incorporate functional groups such as -CH2CONH2 and -CH2CH2CH2NH2. The metal derivatives of these systems show a superficial resemblance to particular biochemical systems such as haemoglobin, in which the metal ion is surrounded by four nitrogen atoms in a square-planar fashion with a fifth donor, attached to another part of the molecule, occupying an axial coordination site. 

In this method the keyhole limpet haemoglobin conjugate was prepared as follows Keyhole limpet haemocyanin (KLH, Calbiochem, La Jolia, CA) and bovine serum albumin (BSA, BDH Chemicals) were coupled to the adduct (2), derived from 6-bromohexanoic acid and monoquat (3), via a carbodiimide reaction, as reported previously by Niewola et al. [184], The resulting conjugates contained 662mol of Paraquat per mole of KLH and 15mol of Paraquat per mole of 6-bromohexanoic acid. The amount of Paraquat bound to the protein was determined by spectrophotometric dithionite assay for Paraquat and the protein concentration was established by a standard Lowry test. 

Figure 3.29 Iso-electric focusing of haemoglobin variants and derivatives. The...

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