Growth Medium and Substrate Effects on Spectroscopic Examination of Cells

4 Growth Medium and Substrate Effects on Spectroscopic Examination of Cells 

A number of studies have investigated the use of FTIR or Raman microspectroscopy as diagnostic tools to differentiate and classify cell lines, in vitro, based on their pathological state or resistance to drugs.Interestingly, we find that some researchers have grown their different cell lines in the same culture media, whereas others have used different media for each cell type. ° The European Collection of Cell Cultures (ECACC) provides standard protocols for the optimum growth of different cell lines. In some instances. 

In a follow-up study, Harvey et al. acquired conventional FTIR spectra from PC-3 cells and LNCaP cells, each grown separately in their optimum culture medium (as advised by ECACC protocols) and a foreign medium .  

Fluorescence assays were conducted at three days post-seeding, as well as fixation using 4% formalin (in PBS) with water rinse for FTIR and Raman investigations. Cellular proliferation, viability and protein content were found to be down-regulated when cells were grown on uncoated quartz compared with uncoated MirrIR substrates. However, increases in proliferation and viability were more pronounced when cells were grown on coated quartz than coated MirrIR substrates. Additionally, it was found that quartz coated with 

Final Time-Lapse Video Frame Brightfield Image of Fixed Area 

---