Time-lapse video

Infants, school-age children, and adolescents have been studied in the laboratory, hospital nurseries, schools, institutions, and their homes with polysomnography, time-lapse video monitors, activity monitors, diaries, and self- and parent-report instruments. Children both with and without sleep disorders have been evaluated with these various assessment procedures, and researchers have used a wide range of study designs to investigate the impact of inadequate sleep. For example, in laboratory studies children and adolescents have been assessed on their usual (often arguably inadequate) schedules as well as on study-defined optimized and restricted... 

Kulesa, P. 1998. Neural crest cell dynamics revealed by time-lapse video microscopy of whole chick explant cultures. Dev. Biol. 204, 327-344. 

Time-lapse video recording is initiated once the slower pump rate is initiated. 

Fig. 2C), and continue time-lapse video recording for an additional 20 min. 

The latter method can also be used in vivo and enables to monitor the process in real time using time-lapse video microscopy. 

Time-lapse video microscopy can be used for tracking physiological processes such as proliferation, infection, tissue reorganization, and the invasion of cancer cells into the healthy tissue. 

Fig. 7. Time-lapse video microscopy of the inner surface of an open-faced explant shows the tendency of mesodermal cells to migrate animally on the inner surface of the prospective neural tissue. As the bulk of the mesoderm convCTges and extends vegetally (arrows. A), a numba- of individual mesodermal cells break off from the posterior edge of the mesoderm and migrate on the inner surface of the prospective neural ectoderm (pointers. A). In some cases, large tongues of mesodermal cells may migrate animally (white arrows, B) while the rest of the mesoderm converges (C) and extends (E) vegetally (black arrows, B). Photos courtesy of John Shih.

Final Time-Lapse Video Frame Brightfield Image of Fixed Area... 

Figure 5.12 (A) Still frames taken from a time-lapse video of prostate cancer cells on...

Cell migration was assayed by automated time-lapse video microscopy. In this technique, cells are imaged on the surfaces and the individual cell tracks are reconstructed from the position of each cell at successive time points. The automation enables time lapse videos to be taken from multiple locations on the same surface at the same time. Thus, the entire gradient can be imaged at once. This high-throughput technique combined with the combinatorial sample preparation enables the exploration of a relatively large parameter space quickly. 

Jensen CG, Jensen LCW, Ault JG, Osorio G, Cole R, Rieder CL. Time-lapse video light microscopic and electron microscopic observations of vertebrate epithelial cells exposed to crocidolite asbestos. In Davis IMG, Jaurand MC, eds. Cellular and Molecular EIFects of Mineral and Synthetic Dusts and Fibres. Berlin Springer-Verlag, 1994 63-78. 

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