Glycosaminoglycan Binding Sites

Lau EK, Paavola CD, Johnson Z, et al. Identification of the glycosaminoglycan binding site of the CC chemokine, MCP-1 implications for structure and function in vivo. J Biol Chem 2004 279 22294-305. 

Possesses a glycosaminoglycan binding site bSerpin domain Kunitz domain... 

Prevents early agent replication in the LRS, competes with GAG (glycosaminoglycan) binding site... 

Koopmann, W, Ediriwickrema, C., and Krangel, M.S. (1999) Structure and function of the glycosaminoglycan binding site of chemokine macrophage-inflammatory protein-1 beta. Journal of Immunology (Baltimore, Md 1950), 163, 2120-2127. 

Koopmann, W. and Krangel, M.S. (1997) Identification of a glycosaminoglycan-binding site in chemokine macrophage inflammatory protein-l-alpha. The Journal of Biological Chemistry, 272, 10103-10109. 

RE Hileman, JR Fromm, JM Weiler, RJ Linhardt. Glycosaminoglycan-protein interactions definition of consensus sites in glycosaminoglycan-binding proteins. Bioessays 20 156-167, 1998. 

Another serine protease inhibitor of the al-antitrypsin family (serpin) is heparin cofactor II (HCII), which also forms a 1 1 complex with thrombin, but does not react with factor Xa [4,10]. The rate of inhibition of thrombin is not only increased by heparinoids but also by the related glycosaminoglycan dermatan sulfate. The identification of an inhibitor variant and site-directed mutagenesis studies on HC II cDNA led to the understanding that the binding sites for heparin and dermatan sulfate may be overlapping but not identical. Further proteinase inhibitors interacting with heparinoids are tissue factor pathway inhibitor and protease nexin-1. 

Raman R, Sasisekharan V, Sasisekharan R. Structural insights into biological roles of protein-glycosaminoglycan interactions. Chem Biol 2005 12 267-277. Esko JD, Selleck SB. Order out of chaos assembly of ligand binding sites in heparan sulfate. Annu Rev Biochem 2002 71 435-471. 

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