Glycoproteins with lentil lectins

All data normalized to D-mannose = 1.0.4F rom Ref. 123 molarity required to produce 50% inhibition of precipitation of lentil lectin with phosphonomannan or glycoprotein. From Ref. 143 minimum amounts (mg/ml) completely inhibiting 4 hemagglutinating doses of lentil lectin, x, no inhibition at 20 mg/ml. "From Ref 213 millimolarity needed to produce 50% inhibition of agglutination. 

Like the jack-bean and pea lectins, the lentil agglutinin interacted with 2-0-(2-acetamido-2-deoxy-j8-D-glucopyranosyI)-D-mannose,210 providing evidence that the lentil lectin binds to internal 2-0-substituted D-mannopyranosyl residues that occur in animal glycoproteins. 

A mixture of lentil lectin-reactive glycoproteins from pig lymphocyte-plasma membrane was isolated by lentil lectin-Sepharose chromatography of sodium deoxycholate-solubilized membranes.445 Eighty-seven percent of the protein applied (17% of hexose) passed through unretarded, and 13% of the applied protein (83% of hexose) was bound, and eluted with methyl a-D-glucopyranoside solution. Recovery was 95% of the material applied, in contrast to the recovery in similar experiments conducted on con A-Sepharose columns (80% recovery).850 The eluate from the lentil column, which contained at least ten glycoproteins, blocked lymphocyte transformation induced by lentil or kidney-bean lectins.445... 

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