Glycoproteins fractionation

Anti-thyroid activity. Boiled rice taken orally by adults at a dose of 350 g/person was inactive on iodine uptake by the thyroid Anti-tumor activity. Bran, administered intraperitoneally to mice at a dose of 100 mg/kg, was active on Sarcoma 180 (solid). The biological activity has been patented . Fermented grains, in the ration of rats, were active. Miso, a paste made from the seeds of Oryza sativa and G lycine max (soybean), was fed ad libitum. The incidence of cancers in the miso treated rats was 20% less than controls vs 7,12-dimethylbenz-[a]anthracene -induced carcinogenesis ". Water extract of dried seed hull, administered intraperitoneally to mice, was active on Sarcoma 180 (ASC). A glycoprotein fraction has been tested. The biological activity reported has been patented . Anti-ulcer activity. A cerebroside fraction of rice bran, administered intraperitoneally to mice at a dose of 100 mg/kg, was inac-... 

Results of an experiment with an oligosaccharide free from amino acid were less conclusive, but were not incompatible with the structure shown. The oligosaccharide was prepared from a separated glycoprotein fraction having a D-mannose hexosamine ratio of 5 3. a-D-Mannosidase and 2-acetamido-2-deoxy-/3-D-glucosidase were used alternately. Initially, a-D-mannosidase removed about 1.5 D-mannose residues per molecule. Subsequent treatment of the residual oligosaccharide with 2-acetamido-2-deoxy-/3-D-glucosidase released rather less than one proportion of hexosamine residue, and then another 1.5 residues of D-mannose became open to attack by a-D-mannosidase. 

Uchida, H., Sasaki, T., Uchida, N. A., Takasuka, N., Endo, Y., and Kamiya, H. (1987). Oncostatic and immunomodulatory effects of a glycoprotein fraction from water extract of abalone, Haliotis discus hannai. Cancer Immunol. Immunother. 24,207-212. 

Kanno, C., Shimizu, M. and Yamauchi, K. 1975. Isolation and physicochemical properties of a soluble glycoprotein fraction of milk fat globule membrane. Agr. Biol. Chem. 39, 1835-1842. 

Keenan, T. W., Powell, K. M., Sasaki, M., Eigel, W. N., and Franke, W. W. 1977B. Membranes of mammary gland. XIV. Isolation and partial characterization of a high molecular weight glycoprotein fraction from bovine milk fat globule membrane. 

Fig. 42.—Structure of the Fundamental, Pentasaccharide Structure of the Major, Glycoprotein Fraction of Hog Submaxillaiy-gland Mucin. 1...

Ten sialic-acid-containing oligosaccharides and four neutral oligosaccharides have been purified from the major glycoprotein fraction of hog, submaxillary-gland mucin. H-N.m.r. spectroscopy at 360 MHz, in combination with methylation analysis and mass spectrometry, allowed the determination of their structures, which are partial structures of a pentasaccharide (see Fig. 42) having blood-group A activity.221... 

Figure 6. Inhibition of fi-galactosidase assimilation by inhibitor glycoproteins, glycopeptides, and derivatives. The open symbols represent data obtained with glycopeptides open squares, inhibitor glycoprotein fraction open circles, unit A glycopeptides prepared from thyroglobulin (30) and open triangle,unit B glycopeptides from thyroglobulin (30).

Choi et al. 86) were able to isolate a glycoprotein fraction (5.5 kDa) that is involved in the wound healing effect of A. vera via cell proliferation and migration. This glycoprotein fraction was able to accelerate the wound heahng on a monolayer of human keratinocytes and also to enhance wound healing in hairless mice with significant cell proliferation. 

W8. Weimer, H. E., and Coggshall, V., Divergent responses of serum glycoprotein fractions to tissue injury in adrenalectomized rats. Can. J. Physiol. Pharmacol. 46, 767-776 (1967). 

The CWM (1 g) is first depectinated by extraction with 0.05 M CDTA at 20°C for 6 h, twice. The insoluble residue is suspended in distilled water (75-100 ml) in a loosely stoppered flask and heated at 70°C. Glacial acetic acid (0.15 ml) and sodium chlorite (0.3 g) are added and the contents stirred for 15 min. The treatment is repeated and the mixture stirred for a further 15 min. To prevent accumulation of chlorine dioxide gas, the contents are continuously flushed with argon. After cooling, the contents are filtered and the residue washed with five bed volumes of distilled water. The filtrate is purged with argon to remove chlorine dioxide, dialyzed, and concentrated to yield the crude chlorite-soluble glycoprotein fraction, and an aliquot is freeze-dried for analysis (yield 80 mg from 1 g dry CWM of runner beans). 

Differences between males and females in the amount of free drug found in plasma, and of protein binding, have been reported for diazepam (Greenblatt et al, 1979 Abel et al, 1979) and for imipramine (Kristensen 1983). In the latter instance, a direct correlation was found with differences in lipoprotein and orosomucoid protein (1-a-acid glycoprotein) fractions (Greenblatt et al,... 

A glycoprotein (mol. wt, 1.5 X 10 ) has been isolated from whole human platelets by affinity chromatography on immobilized wheat-germ agglutinin. Extraction of the whole platelets with lithium di-iodosalicylate released three major glycoprotein fractions. 

When isolated brush borders from rabbit small intestine were incubated with Na2 S04 hydrophobic glycolipids were found to contain 24% of the incorporated and 73% was found in the glycoprotein fraction. One principal, sulphated glycolipid that was present contained D-glucose, D-galactose, and 2-acetamido-2-deoxy-D-glucose (in the molar ratio 1 2 2) but was devoid of neuraminic acid residues. 

Affinity chromatography of glycoprotein fractions 180 from long-term cultured human lymphocytes... 

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