Glycoproteins anionic

Lectins (proteins and/or glycoproteins of non-immune origin that agglutinate cells, from seeds of Robinia pseudoacacia), M 100,000. Purified by pptn with ammonium sulfate and dialysis then chromatographed on DE-52 DEAE-cellulose anion-exchanger, hydroxylapatite and Sephacryl S-200. [Wantyghem et al. Biochem J 237 483 1986.]... 

The anion exchange protein (band 3) is a transmembrane glycoprotein, with its carboxyl terminal end on the external surface of the membrane and its amino terminal end on the cytoplasmic surface. It is an example of a multipass membrane protein, extending across the... 

Reddy, G. P. and Bush, C. A., High-performance anion exchange chromatography of neutral milk oligosaccharides and oligosaccharide alditols derived from mucin glycoprotein, Anal. Biochem., 198, 278, 1991. 

Because of their interference with the interaction between the viral envelope gpl20 glycoprotein and the cellular CD4 receptor, poly anionic substances not only inhibit virus adsorption to the cells but also block syncytium (giant cell) formation between the HIV-infected (gpl20 + ) cells and uninfected (CD4 + ) cells. Since syncytium formation results in a selective destruction of the CD4+ cells, this syncytium formation may play an important role in the pathogenesis of AIDS (a hallmark of which is a progressive decline of the CD4+ cells). 

The anion of nitromethane adds easily to the carbonyl functions of sugars. This is a useful strategy for extension of the carbon chain.100 2-Acetamido-2-deoxy-P-D-glucose (A-acetyl-D-glucosamine) is the carbohydrate unit of glycoproteins that occurs most often. The nitromethy-lation method provides a straightforward route to a series of C-glycosyl compounds with the acetamido functionality (Eq. 3.62).101... 

There have been several studies that underscore the importance of unbound concentration in cell-based studies of receptor function. In a model study of the effect of plasma protein binding on the renal transport of organic anions using the expression of various organic anion transporters (OATPs) in Xenopus oocytes, the transport of ochratoxin A, methotrexate, and estrone sulfate was found to be strongly inhibited by the addition of human serum albumin to the culture medium [16]. Similarly, the addition of oq-acid glycoprotein was found to reverse the blockade of sodium-ion current by cocaine in a preparation of cardiac myocytes [17]. 

Peripheral to the Gal-GlcNAc sequences of glycoproteins, there can occur an array of blood-group-related glycosylations, anionic substitutions (sialy-lation, 0-sulfation, and 0-phosphorylation), or both. Chains of the high-mannose type may also bear phosphate groups. Table VI reviews the pat-... 

Patterns of Peripheral Anionic Glycosylation Documented in O- and N-Linked Chains of Mammalian Glycoproteins ... 

Yamazaki, M., Li, B., Louie, S.W., Pudvah, N.T., Stocco, R., Wong, W., Abramovitz, M., Demartis, A., Laufer, R., Hochman, J.H., Prueksaritanont, T. and Lin, J.H. (2005) Effects of fibrates on human organic anion-transporting polypeptide 1B1-, multidrug resistance protein 2- and P-glycoprotein-mediated transport. Xenobiotica, 35, 737-753. 

Sugiyama, Y., Kato, Y., and Chu, X. (1998) Multiplicity of bdiary excretion mechanisms for the camptothecin derivative irinotecan (CPT-11), its metabolite SN-38, and its glucuronide role of canalicular multispecific organic anion transporter and P-glycoprotein. Cancer Chemother. Pharmacol. 42(suppl), S44-S49. 

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