Glycoconjugates probes

Manger, I.D., Rademacher, T.W., and Dwek, R.A. (1992) 1-N-Glycyl P-oligosaccharide derivatives as stable intermediates for the formation of glycoconjugate probes. Biochemistry 31, 10724-10732. 

R268 Y. Yamaguchi and K. Kato, Dynamics and Interactions of Glycoconjugates Probed by Stable-Isotope-Assisted NMR Spectroscopy , in Methods in Enzymology, ed. M. Fukuda, Elsevier Inc., 2010, Vol. 478, Glycomics, p. 305. 

Biotinylated oligosaccharides are convenient probes of carbohydrate interactions, because the biotin label can be captured or detected using an avidin or streptavidin derivative. For instance, immobilized streptavidin can be used to purify glycoconjugates that have been labeled... 

Other molecules can be used in this type of assay approach. Hydrazide-modified (strept)avidin, lectins, biocytin, fluorescent probes and other detectable molecules can be used to detect specifically glycoconjugates in biological samples (Wilchek and Bayer, 1987). 

R. R. Schmidt, Chemical synthesis of silylated glycoconjugates, in Synthetic Oligosaccharides-Indispensible Probes for the Life Sciences, P. Kovac, ed., American Chemical Society, Washington DC, 1994, p. 276. 

Carbohydrate-directed antibodies raised against neoglycoproteins have been used as probes for the presence and function of cell-surface glycoconjugates,135 in much the same way as are lectins138 and natu-... 

Lectins, or proteins with specific binding sites for carbohydrates, can be used as targeting molecules to localize particular glycoconjugates such as glycoproteins or glycolipids on cell surfaces (Fig. 373). Labeled with gold particles, lectins are important probes for detection of cell surface components and intracellular receptors and in immunological or biochemical assay procedures (Bog-Hansen et al., 1978 Kimura et al., 1979 Nicolson, 1978 Roth, 1983 Benhamou et al., 1988 Nakajima et al., 1988). 

Ogura H, Hasegawa A, Suami T (eds) (1992) Carbohydrates. Synthetic methods and applications in medicinal chemistry. VCH and Kodansha, Weinheim and Tokyo Kovac P (ed) (1994) Synthetic oligosaccharides. Indispensable probes for the life sciences. ACS Symposium Series 560. American Chemical Society, Washington Whitfield DM, Douglas SP (1996) Glycoconjugate J 13 5... 

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