Oligosaccharides biotinylation

Biotinylated oligosaccharides are convenient probes of carbohydrate interactions, because the biotin label can be captured or detected using an avidin or streptavidin derivative. For instance, immobilized streptavidin can be used to purify glycoconjugates that have been labeled... 

Rothenberg, B.E., Hayes, B.K., Toomre, D., Manzi, A.E., and Varki, A. (1993) Biotinylated diaminopyri-dine An approach to tagging oligosaccharides and exploring their biology. PNAS 90, 11939-11943. 

Melrose, J., Tammi, M., and Smith, S., Visualisation of hyaluronan and hyaluronan-binding proteins within ovine vertebral cartilages using biotinylated aggrecan Gl-link complex and biotinylated hyaluronan oligosaccharides, Histochem. Cell Biol., 2002 117, 327, 2002. 

Chen VJ, Wold F. Neoglycoproteins preparation and properties of 49. complexes of biotinylated asparagine-oligosaccharides with avidin... 

Then they reported an efficient and stereocontrolled construction of homo- and heterogeneously 4-and 6-sulphated CS oligomers as their biotinylated conjugates from suitable protected disaccharide building blocks. A series of sulphated and unsulphated analogues of the linkage oligosaccharide and of the constitutive unit of CS has been synthesized. ... 

Streptavidin-dextran Biotin Biotinylated molecules like Oligosaccharides or nucleic acids... 

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