Glycerol as a carbon source

Active compounds were retested in medium containing glycerol as a carbon source. Glycerol does not require PFK for introduction into bacterial energy metabolism pathways. Compounds that were as toxic to the recombinant bacteria in the presence of glycerol as they were in mannitol could not be acting via inhibition of PFK. Conversely, compounds that are significantly more toxic in... 

Several microorganisms could be adapted or engineered to consume glycerol as a carbon source for the production of chemicals that could be used either as end products or as important building blocks. Compared to saccharides, there are... 

Lee, P.C., Lee, W.G., Lee, S.Y. and Chang, H.N. 2001. Succinic Acid Production with Reduced By-Product Formation in the Fermentation of Anaerobiospirillum Succiniciproducens Using Glycerol as a Carbon Source. Biotechnol. Bioeng., 72, 41 —48. 

Many bacteria can use glycerol as a carbon source. As it is a by-product of the production of bio-diesel it can be achieved chet ly. Therefore it seems to be a good alternative to glucose for die production of Polyhydroxyalkanoates. Sodiumvalerate is often used as a precursor in order to obtain the copolymer Poly-3-hydroxybutyrate-co-3-hydroxyvalerate. As Biorelated Potymers Sustainable Polymer Science and Technology Edited by Chiellini et al., Kluwer Academic/Plenum Publishers, 2001 147... 

Lee PC, Lee WG, Lee SY, Chang HN (2001) Succinic acid production with reduced by-product formation in the fermentation of AnaerobiospiriUum succiniciproducens using glycerol as a carbon source. Biotechnol Bioeng 72 41 8... 

One medium (designated medium 151b) has been described by Ghisalba et al. (49) that contains (g/liter) glucose 70 glycerol 20 meat meal 30 soybean meal 10 CaCOj 8 (NH )jSO, 3 KH PO 1 trace amounts of Fe, Cu, Zn, Mn Co , and This medium differs from those reported by Sensi and Thiemann (70) for the industrial production of rifamycin B mainly ii the presence of glycerol as a carbon source. 

A. Bzducha-Wrobel, M. Kieliszek, S. Blazejak, Chemical composition of the cell wall of probiotic and brewers yeast in response to cultivation medium with glycerol as a carbon source, Eur. Food Re. Technol., 237,489-499, 2013. 

VAPs and its recycling into lipids and/or fats by various microbes. Biotechnological processes have been studied extensively for years, wifh a number of differenf microorganisms, to use glycerol as a carbon source for bioconversions. Some of the useful chemicals thaf can be produced by bioconversions include 1,3-PDO, succinic acid, citric acid, and PHAs (Koutinas et al., 2007), while the production of other chemicals is still being explored. 

Ryu, H.W, Kang, K.H., Yun, J.S., 1999. Bioconversion of fumarate to succinate using glycerol as a carbon source. Applied Biochemistry and Biotechnology 77-79, 511-520. 

Meesters, P.A.E.P., Huijberts, G.N.M., Eggink, G., 1996. High cell density cultivation of the lipid accumulating yeast Cryptococcus curvatus using glycerol as a carbon source. Applied Microbiology and Biotechnology 45, 575—579. 

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