Glycan affinity chromatography

TABLE 3.7 Specificity of some lectins used in glycan affinity chromatography... 

Analytical tools have been developed in order to identify carbohydrate structures as well as carbohydrate-binding proteins and to understand their underlying structure-function relationships of protein-carbohydrate and carbohydrate-carbohydrate interactions lectin arrays [16], glycan microarrays [17, 18], glyco-nanoparticles [19], frontal affinity chromatography [20] and carbohydrate tools for metabolic labeling [21]. 

N-glycans can be isolated by means of conventional lectin-based affinity chromatography, as described previously. 

Biotin-labeled glycan tf HRP-avidin Affinity chromatography 16... 

Naka, R., Kamoda, S., Ishizuka, A., Kinoshita, M., Kakehi, K. (2006). Analysis of total A-glycans in cell membrane fractions of cancer cells using a combination of serotonin affinity chromatography and normal phase chromatography. 7. Proteome Res., 5, 88-97. 

Lyon and Phelps [57] have evaluated various glycan-substituted AH-Sepharose gels to be used for affinity chromatography. Srivastava and Farooqui reported the elution of hyaluronidase from heparin-Sepharose columns using heparin or hyaluronan solutions as eluent [58]. Alternatively, affinity chromatography of hyaluronidase has been performed using concavalin A-Sepharose [58] or Matrex gel phenyl boronate [59] columns. 

Suitable pairs of protein and ligand combination for affinity chromatography are antigen-antibody, hormone-receptor, glycoprotein-lectin or enzyme-substrate/cofactor/ effector (Table 3.6). The affinity of lectins for specific carbohydrate moieties (Lis and Sharon, 1973 Barondes, 1981) has made them particularly useful for the purification of distinct groups of glycans and glycoproteins (Table 3.7). 

Sato C, Yamakawa N, Kitajima K (2010) Measurement of glycan-based interactions by frontal affinity chromatography and surface plasmon resonance. Methods Enzymol 478 219-232... 

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