Glucosinolate biosynthesis

In the last 40 years, a variety of classical approaches, including precursor feeding experiments, enzymological investigations, and genetic studies have been employed to elucidate the general pathway of glucosinolate biosynthesis. Recently, 

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BAK, S., TAX, F.E., FELDMANN, K.A., GALBRAITH, D.W., FEYEREISEN, R., CYP83B1, A cytochrome P450 at the metabolic branch point in auxin and indole glucosinolate biosynthesis in Arabidopsis, Plant Cell, 2001,13,101-111. 

Cytochromes P450 of the CYP79 Family in Glucosinolate Biosynthesis.227... 

CYTOCHROMES P450 OF THE CYP79 FAMILY IN GLUCOSINOLATE BIOSYNTHESIS... 

Besides its role as an intermediate in indole glucosinolate biosynthesis, it has been suggested that IAOX is involved in IAA biosynthesis.40,41 This has been supported by the biochemical characterization of LAOX-metabolizing enzymes that convert IAOX to either indole-3-acetaldehyde,42,43 indole-3-acetonitrile,42,44 or... 

Biochemical Characterization of the Oxime-Metabolizing Enzyme in Glucosinolate Biosynthesis... 

GLENDENING, T.M., POULTON, J.E., Glucosinolate biosynthesis. Sulfation of desulfoglucosinolate by cell-free extracts of cress (Lepidium sativum L.) seedlings, Plant Physiol., 1988,86, 319-321. 

DU, L., LYKKESFELDT, J, OLSEN, C.E., HALKIER, B.A., Involvement of cytochrome P450 in oxime production in glucosinolate biosynthesis as demonstrated by an in vitro microsomal enzyme system isolated from jasmonic acid-induced seedlings oiSinapis alba L, Proc. Natl. Acad. Sci. USA, 1995, 92, 12505-12509. 

Glucosinolate biosynthesis further characterization of the aldoxime-forming microsomal monoxygenases in oilseed rape leaves, Plant Physiol., 1995, 109, 299-305. 

RODMAN, J.E., SOLTIS, P.S., SOLTIS, D.E., SYTSMA, K.J., KAROL, K.G., Parallel evolution of glucosinolate biosynthesis inferred from congruent nuclear and plastid gene phylogenies, Amer. J. Bot., 1998,85,997-1006. 

BAK, S., FEYEREISEN, R., The involvement of two P450 enzymes, CYP83B1 and CYP83A1, in auxin homeostasis and glucosinolate biosynthesis, Plant Physiol., 2001,127, 108-118. 

Hirai MY, Sugiyama K, Sawada Y, Tohge T, Obayashi T, Suzuki A, Araki R, Sakurai N, Suzuki H, Aoki K, Goda H, Nishizawa OI, ShibataD, Saito K (2007) Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis. Proc Natl Acad Sci USA 104 6478-6483... 

Biosynthesis of Polyketides Phenolic Compounds derived from Shikimate The Biosynthesis of C5—C20 Terpenoid Compounds Triterpenoids, Steroids, and Carotenoids Non-protein Amino-acids, Cyanogenic Glycosides, and Glucosinolates Biosynthesis of Alkaloids. 

KLIEBENSTEIN, D.J., LAMBR1X, V.M., REICHELT, M., GERSHENZON, J., MITCHELL-OLDS, T., Gene duplication in the diversification of secondary metabolism Tandem 2-oxoglutarate-dependent dioxygenases control glucosinolate biosynthesis in Arabidopsis., Plant Cell, 2001,13,681-693. 

Elongation of amino acid side chains prior to glucosinolate biosynthesis has been studied in several plants. The mechanisms involved are believed to be similar to the formation of leucine from valine and acetate (Fig. 3.13). Through transamination, the amino acid is converted to the corresponding... 

Bennett, R.N., Dawson, G.W., Hick, A.J. and Wallsgrove, R.M. (1995b) Glucosinolate biosynthesis further characterization of the aldoxime-forming microsomal monooxygenases in oilseed rape leaves. Plant Physiol., 109, 299-305. 

Du, L. and Halkier, B.A. (1996) Isolation of a microsomal enzyme system involved in glucosinolate biosynthesis from seedlings of Tropaeolum majus (L.). Plant Physiol, 111, 831-7. 

Grubb, G.D., Zipp, B.J., Ludwig-Muller, J., Masuno, M.N., Molinski, T.F. and Abel, S. (2004) Arabidopsis glucosyltransferase UGT74B1 functions in glucosinolate biosynthesis and auxinhomeostasis. Plant., 40, 893-908. 

Jain, J.C., GrootWassink, J.W.D., Reed, D.W. and Underhill, E.W. (1990b) Persistent copurification of enzymes catalyzing the sequential glucoxylation and sulfation step in glucosinolate biosynthesis. /. Plant Physiol, 136, 356-61. 

Mikkelsen, M.D., Naur, P. and Halkier, B.A. (2004) Arabidopsis mutants in the C-S lyase of glucosinolate biosynthesis establish a critical role for indole-3-acetaldoxime in auxin homeostasis. Plant., 37, 770-77. 

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