Mutants Arabidopsis

It has been important to determine if the neoxanthin distortion signature could be detected during the nonphotochemical quenching in vivo. Resonance Raman measurements on leaves and chlo-roplasts of various Arabidopsis mutants have revealed a small increase in the 950 cm 1 region. The relationship between the amplitude of this transition and the amount of NPQ suggests that the LHCII aggregation may be the sole cause of the protective chlorophyll fluorescence quenching in vivo (Ruban et al., 2007). 

DORMANN, P., HOFFMANN-BENNING, S., BALBO, I., BENNING, C., Isolation and characterization of an Arabidopsis mutant deficient in the thylakoid lipid digalactosyl diacylglycerol, Plant Cell, 1995, 7, 1801-1810. 

TANAKA, A., TANO, S., CHANTES, T., YOKOTA, Y., SHIKAZONO, N., WATANABE, H., A new Arabidopsis mutant induced by ion beams affects flavonoid synthesis with spotted pigmentation in testa, Genes Genet. Syst., 1997, 72, 141-148. 

M., AUSUBEL, F.M., GOODMAN, H., Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis, Plant J., 1995, 8, 659-671. 

BIEZA, K., LOIS, R., An Arabidopsis mutant tolerant to lethal ultraviolet-b levels shows constitutively elevated accumulation of flavonoids and other phenolics, Plant Physiol., 2001,126, 1105-1115. 

Lois R, Buchanan BBN (1994) Severe sensitivity to ultraviolet radiation in an Arabidopsis mutant deficient in flavonoid accumulation II. Mechanisms of UV-resistance in Arabidopsis. Planta 194 504-509... 

Ormrod, D.P., Landry, L.G., and Conklin, P.L., Short-term UV-B radiation and ozone exposure effects on aromatic secondary metabolite accumulation and shoot growth of flavonoid-deficient Arabidopsis mutants, Physiol Plant., 93, 602, 1995. 

Landry, L.G., Chappie, C.C.S., and Last, R.L., Arabidopsis mutants lacking phenolic sunscreens exhibit enhanced ultraviolet-B injury and oxidative damage. Plant Physiol, 109, 1159, 1995. 

Another Arabidopsis mutant, murl, which lacks the ability to synthesize 1-fucose, possesses a defective gene encoding GDP-d-Man-4,6-dehydratase, a key enzyme in 1-fucose biosynthesis. Further analysis revealed that 1-Fuc is replaced by 1-Gal, a structurally similar monosaccharide, in the cell walls of this mutant with no adverse effects on plant physiology or metabolism (Rayon et al, 1999). Transgenic plants containing this mutation can also be used for foreign protein production. 

Recent studies, using an Arabidopsis mutant defective in the xanthophyll cycle, point to a chlorophyllbinding protein PsbS, which participates in nonphoto-... 

The Arabidopsis mutant dwf7/ste 1 is defective in C5-desaturation of episterol (Fig. (2)) [18], thus impaired in an enzyme function involved in a very early step of brassinosteroid precursor biosynthesis. The enzymatic block of dwf7/stel was determined by feeding experiments using l3C-labelled mevalonic acid and a subsequent analysis of endogenous sterol and brassinosteroid precursors. The mutant accumulates episterol with a simultaneous decrease of downstream intermediates (24-methylenecholesterol, campesterol, castasterone, brassinolide). 

Booij-James IS, Dube SK, Jansen MAK, Edelman M, Mattoo AK. 2000. Ultraviolet-B radiation impacts light-mediated turnover of the photosystem II reaction center heterodimer in Arabidopsis mutants altered in phenolic metabolism. Plant Physiol 124 1275-1284. 

Lloyd JC, Zakhleniuk OV. 2004. Responses of primary and secondary metabolism to sugar accumulation revealed by microarray expression analysis of the Arabidopsis mutant, pho3. J Exp Bot 55 1221-1230. 

The important role of sterol components in the acquisition of freezing tolerance has recently been demonstrated using an Arabidopsis mutant with altered steryl-ester metabolism. Compared with the wild-type the mutant has no visible phenotype at standard growth temperature but exhibits clear symptoms when exposed to low temperatures (Hugly et al.,... 

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