Glucosidases p-glucosidase

The enzymes used included cellulase, a-glucosidase, P-glucosidase, mannanase, and hemicellulase. The following four cellulase samples were... 

In order to account for the inability of many enzymes to bind the protonated form of the basic inhibitors or permanently cationic ones better than uncharged analogs (for example, yS-o-galactosidase from E. coli, and P-v>-glucosidase from almonds), it was proposed that the enzyme could proton-ate the inhibitor at the active site by a cationic acid (for example, protonated histidine). If proton transfer cannot occur, the attractive forces due to the carboxylate would be canceled by the repulsion from the cationic acid. Experimental evidence for this proposal is, however, still lacking. In fi-D-gn-lactosidase from E. coli, a tyrosine is presumed to be responsible for the protonation of substrates. ... 

Potential enzymes involved in anthocyanin metabolism — The lactase phlorizin hydrolase (LPH EC 3.2.1.108) present only in the small intestine on the outside of the brush border membrane and the cytosolic P-glucosidase (CBG EC 3.2.1.1) found in many tissues, particularly in liver, can catalyze the deglycosylation (or hydrolysis) of polyphenols. LPH may play a major role in polyphenol metabolism... 

Two main groups can be discerned (1) endogenous factors such as plant enzymes like polyphenoloxidase, peroxidase, and P-glucosidase and (2) conditions prevailing in the extraction medium will decide the fates of betalain pigments among which temperature, oxygen, and pH are considered the most important conditions. 

Synthesis of naturally occurring (J-D-glucopyranosides based on enzymatic P-glucosidation using -glucosidase from almond... 

Different types of gel materials, such as polysaccharides, proteins and synthetic polymers, are now used to entrap biocatalysts. Among them, photo-crosslinkable resin prepolymer ENTP-4000 as shown in Eig. 7 is more useful compared to others. Entrapment of biocatalysts should be carried out under the illumination of near ultraviolet hght within 3-5 min, by which high temperatures, shifts of pH to extremely alkahne or acidic sides are avoided. ENTP-4000, hydrophobic photo-crosslinkable resin prepolymer, is one of the most suitable prepolymers for entrapment of p-glucosidase. Molecular weight of its main chain is about 4000. 

Protein isolation with affinity precipitation has been discussed in detail by Mattiasson and co-workers (see, e.g. Galaev and Mattiassion, 1997) and they have provided an exhaustive tabulation. Polymers varied from alginate.s/chitosan to dextran to NIPAM. Examples of the used proteins are from trypsin, p-glucosidase, xylanase, alkaline protease, etc. It is remarkable that affinity precipitation can sometimes give results comparable to affinity chromatography. 

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