Cellulases P-glucosidase

Index Entries Cellulose cellulase P-glucosidase Orpinomyces cellobiase. 

Hydrolysis of Ammonia-pretreated Sugar Cane Bagasse with Cellulase, P-Glucosidase, and Hemicellulase Preparations... 

Keywords Saline Creeping Wild Ryegrass Cellulase P-Glucosidase Tween 20 Tween 80 Bovine serum albumin - Enzymatie hydrolysis Avicel PH 101-Lignaceous residue... 

Two commercials enzymes, Celluclast 1.5-L and Novozyme 188 (Sigma, St Louis, MO, USA), were used for enzymatic hydrolysis. Celluclast 1.5-L contained 98.2 FPU/ml of total cellulase P-glucosidase activity of Novozyme-188 was 540 unit/ml. One FPU is defined as the enzyme amount that releases 1 p.mol of glucose equivalents from Whatman no. 1 filter paper in 1 min. One unit of 3-glucosidase activity is defined as the enzyme amount that converts 1 imol of cellubiose to 2 pmol of glucose in 1 min [17],... 

Index Entries Cellulase production Trichoderma reesei RUT C30 pH profiling p-glucosidase shake flask fermentor. 

The cellulase and P-glucosidase activities were 30 filter paper units/g of DM substrate ... 

Foam fractionation is a promising technique for concentrating proteins because of its simplicity and low operating cost. One such protein that can be foamed is the enzyme cellulase. The use of inexpensively purified cellulase may be a key step in the economical production of ethanol from biomass. We conducted foam fractionation experiments at total reflux using the cellulase component P-glucosidase to study how continuous shear affects P-glu-cosidase in a foam such as a fermentation or foam fractionation process. The experiments were conducted at pH 2.4, 5.4, and 11.6 and airflow rates of 3,... 

Index Entries P-Glucosidase foam fractionation cellulase reflux time airflow rate. 

Activities for cellulase (FPU/mL) and P-glucosidase (IU/mL) were measured by the methods described previously (16,17). ODs (1-cm light path) of cultures were monitored on a Beckman DU-640 Spectrophotometer (Fullerton, CA) at 550 (E. coli) or 660 nm (S. cerevisiae). Concentrations of sugars and ethanol were determined by HPLC using an Aminex HPX-87H column (300 x 7.8 mm Bio-Rad, Richmond, CA) and refractive index detector. Samples were run at 65°C and eluted at 0.6 mL/min with 5 mM H2S04. 

Commercial cellulase and P-glucosidase (Novo Nordisk, Bagvaerd, Denmark) supplied from Novozymes Korea were used. A mixture of Celluclast (80 IU or international filter paper units [IFPU]/mL) and Novozym 188 (792 cellobiase units [CBU]/mL) was used with a ratio of 4 IU of Celluclast/CBU of Novozym to alleviate end-product inhibition by cellobiose. 

The enzyme preparations were provided by Novo Nordisk. Cellulase (Celluclast) possessed 115 filter paper units (FPU)/mL P-glucosidase (Novozym 188) had 570 cellobiose units (CBU)/mL. 

All hydrolysis experiments were conducted with a combination of cellulase (Celluclast) and P-glucosidase (Novozym 188) at a ratio of 1 2... 

Index Entries P-Glucosidase cellulase degree of inhibition softwood glucose hydrolysate. 

In the present study, we quantified the degree of inhibition on both P-glucosidase and cellulase mixtures by glucose and cellobiose at different concentrations. We also determined the inhibitory effects of mannose, galactose, and xylose on both P-glucosidase and cellulase activities and assessed the potential to increase the final sugar concentration by supplementing cellulosic substrate hydrolysis with hemicellulose-rich stream-obtained from steam exploded softwood (prehydrolysate). 

Fig. 4. Comparison of degree of glucose inhibition on cellulase and P-glucosidase activities during hydrolysis of cellulose and cellobiose at two substrate consistencies 2 and 10% (w/v). Cel, cellulase P-G, P-glucosidase.

Gunjikar, T. P., Sawant, S. B., and Joshi, J. B. 2001. Shear Deactivation of Cellulase, Exoglucanase, Endoglucanase, and P-Glucosidase in a Mechanically Agitated Reactor. Biotechnol. Prog., 17,1166-1168. 

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