Of P-glucosidase

Different types of gel materials, such as polysaccharides, proteins and synthetic polymers, are now used to entrap biocatalysts. Among them, photo-crosslinkable resin prepolymer ENTP-4000 as shown in Eig. 7 is more useful compared to others. Entrapment of biocatalysts should be carried out under the illumination of near ultraviolet hght within 3-5 min, by which high temperatures, shifts of pH to extremely alkahne or acidic sides are avoided. ENTP-4000, hydrophobic photo-crosslinkable resin prepolymer, is one of the most suitable prepolymers for entrapment of p-glucosidase. Molecular weight of its main chain is about 4000. 

V. Patel and A. L. Tappel, Identity of P-glucosidase and P-xylosidase activities in rat liver lysosomes, Biochim. Biophys. Acta Enzymol., 191 (1969) 653-662. 

S. G. Withers and K. Umezawa, Cyclophellitol A naturally occurring mechanism-based inactivator of P-glucosidases, Biochem. Biophys. Res. Commun., 177 (1991) 532-537. 

T. M. Wrodnigg, A. E. Stiitz, C. A. Tarling, and S. G. Withers, Fine tuning of p-glucosidase inhibitory activity in the 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) system, Carbohydr. Res., 341 (2006) 1717-1722. 

What is the activity (units cnr () of P-glucosidase in the enzyme solution ... 

Figure 3. Development of p-glucosidase activity. Same conditions as Figure 2. ( )) Whole culture broth (O) culture filtrate ( ) culture solids...

The palatability of safflower meal to poultry tends to be low (Ravindran and Blair, 1992) due to the presence of two phenolic glucosides matairesinol-p-glucoside, which imparts a bitter flavour, and 2-hydroxyarctiin-p-glucoside, which also has a bitter flavour and has cathartic properties (Darroch, 1990). Both glucosides are associated with the protein fraction of the meal, and they can be removed by extraction with water or methanol, or by the addition of P-glucosidase. 

The amounts of P-glucosidase were below the expected values and an activity of about 0.4 IU/mL was achieved in all cases. In each experiment, the amount of P-glucosidase increased steadily throughout the whole fermentation, reaching a maximum at h 60. No noteworthy differences in the production rates were seen. 

Fig. 1. Schematic of apparatus for foam fractionation of P-glucosidase at total reflux.

Rosi, I., Vinella, M., and Domizio, P. (1994). Characterization of P-glucosidase activity in yeasts of oenological origin. J. Appl. Bacteriol. 77, 519-527. 

Yeoh, H.-H. (1989) Kinetic properties of p-glucosidase from Cassava. Phytochemistry, 28, 721-4. 

HomoDMDP-7-O-P-D- xylopyranoside potent inhibitor of P-glucosidases and p-galactosidases (including lactase) and moderate inhibitor of amyloglucosidase and P-mannosidase ... 

Broussonetines E and F potent inhibitors of P-glucosidase, P-galactosidase and p-mannosidase and good inhibitors of a-glucosidase ... 

Hyacinthacine A2 Potent inhibitor of amyloglucosidase, good inhibitor of lactase, moderate inhibitor of P-glucosidase and trehalase and weak inhibitor of 3-mannosidase ... 

Calystegine Bi potent inhibitor of P-glucosidases (including cellobiase) and -galactosidase (including lactase) and a weak inhibitor of trehalase ... 

Calystegine B -3-0-p-D-glucopyranoside potent inhibitor of rice a-glucosidase and weak inhibitor of p-glucosidases" ... 

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