Glucose-amino acid systems mutagenic activity

Table III. Mutagenic activity expressed as revertants per plate (TA98+S9) of model reaction systems (creatinine, glucose, amino acid in diethylene glycol-water 6 1, v/v). Samples in triplicates were directly withdrawn from the mixtures after 1, 2 and 4 h of reflux. 50 pi were used per plate. Comparison of mutagenic activity after corrections for spontaneous revertants between model mixtures containing alanine or glycine.

Figure 4. Effect of reflux time, amino acid (glycine or alanine), and Salmonella strain on the mutagenic activity formed in model reaction systems of creatinine (1.25 g), o-glucose (0.8 g), and amino acid (1.07 g) in diethylene glycol-water (6 1 v/v).

Thus, by the Maillard reaction in different browning systems of sugars and amino compounds, some mutagenic substances were formed, although their activities are quite weak compared with those formed by pyrolysis of amino acids. They were confirmed as intermediates and some of them were identified as furan, pyrrole, or thiazolidine derivatives formed from glucose and amino acids... 

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