Glucanases differentiation

Two cell-wall fractions were obtained from mycelia of Neurospora crassa by differential extraction with alkali (soluble in 4%, and insoluble in 24%) on incubation with endo-(l - 3)-/3-D-glucanase, both gave473 D-glucose, laminara-biose and -triose, and 62-/3-D-glucosyllaminarabiose (118). exo-(1 - 3)-/3-D-Glucanase released D-glucose and gentiobiose. Structures were proposed for both fractions when this evidence and the results of methylation analysis were considered. 

Ward, E., Payne, G., Moyer, M., Williams, S., Dincher, S., Sharkey, K., Beck, J., Taylor, H., Ahl-Goy, P., Meins, F. Ryals, J. (1991). Differential regulation of P-l,3-glucanase mRNAs in response to pathogen infection. Plant Physiology 96, 390-7. 

SALZER, P., HUBNER, B., SIRRENBERG, A., HAGER, A., Differential effect of purified spruce chitinases and I3-l,3-glucanases on the activity of elicitors from ectomycorrhizal fungi.. Plant Physiol., 1997,114, 957-968. 

The ability of yeast cells to liberate sphaeroplasts following treatment with (1-> 3)-/3-D-glucanases has been proposed as a taxonomic criterion for differentiating basidiomycetous from ascomycetous yeasts and for classifying the yeasts of the fungi imperfecti. ... 

Lambais, M.R. and Mehdy, M.C. (1996) Soybean roots infected by Glomus intraradices strains differing in infectivity exhibit differential chiti-nase and P-l,3-glucanase expression. New Phytol 134, 531-538... 

Yeast-cell lytic enzymes have been differentiated serologically, using antibodies elicited towards an e fito-jS-l,3-D-glucanase from a species of Fungiimperfecti this technique revealed the absence of any immunological common structure. ... 

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