Glucanases activity

Table II. Effects of xyloglucan oligosaccharide subunits on auxin-stimulated growth of pea epicotyl segments in vivo and pea endo-1,4-/ -glucanase activity in vitro. Growth of segments in 1 //M 2,4-D was measured after 18 h. Endo-1,4-/J-gIucanase activity was determined viscometrically after 30 min using tamarind xyloglucan as substrate. Data calculated from refs. 50 and 51...

Makarieva, T.N. Stonik, V.A. lyyachuk, O.G. Dmitrenok, A.S. (1995B) Annastool sulfate, a novel marine sul ted sto-oid, inhibitor of glucanase activity from a deep wato sponge, Poecillastra laminaris. Tetrahedron Lrtt., 36,129-32. 

Kauffmann, S., Legrand, M., Geoffrey, P. Fritig, B. (1987). Biological function of pathogenesis-related protein four PR proteins of tobacco have l,3p glucanase activity. The EMBO Journal 11,3209-12. 

Application and Principle This procedure is used to determine 6-glucanase activity of enzyme preparations derived from Aspergillus niger var. and Bacillus subtilis var. The assay is based on a 15-min hydrolysis of lichenin substrate at 40° and at pH 6.5. The increase in reducing power due to liberated reducing groups is measured by the neocuproine method. 

Wine lactic acid bacteria can degrade polysaccharides and O. oeni has been shown to have an extracellular (3 (l->3) glucanase activity (Guilloux-Benatier et al. 2000). 

Recent developments in enzyme manufacturing have led to commercial products that contain a preponderance of one cellulase component. These mono-component enzymes are produced from modified Humicola strains and are primarily endo-glucanases active at pH 7-7.5 and are referred to as alkaline cellulases . 

Boschker H. T. S. and Cappenberg T. E. (1994) A sensitive method using 4-methylumbelliferyl- 3-cellobiose as a substrate to measure (L4)- 3-glucanase activity in sediments. Appl. Environ. Microbiol. 60, 3592-3596. 

Abd-El-Al and Phaff (39) also found -glucanase activities in intracellular extracts of Hanseniaspora valbyensis and Hanseniaspora uvarum and in the culture fluid of H. valbyensis. In contrast to the other yeasts, pustulan was not hydrolyzed by these preparations, and laminarin was hydrolyzed yielding oligosaccharides. This suggested that these yeasts produced an endo-/3- (1 — 3) -glucanase, and subsequently such an enzyme was partially purified from the culture fluid of H. valbyensis (39). 

Evidence for the presence of endo-/3-( 1 - 3)-glucanase in multi-la terally budding yeasts was first supplied in 1970 by Bacon et al. (43) who mentioned briefly that such an enzyme was associated with cell walls of bakers yeast. Maddox and Hough (50) detected endo-/ -(l - 3-glucanase activity in chloroform-induced autolyzed brewer s yeast, while... 

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