Glucanases cellulose activity

Carbohydrase [(Trichoderma longibrachiatum var.) (formerly reesei)] Produced as an off white to tan, amorphous powder or as a liquid by controlled fermentation using Trichoderma longibrachiatum var. Soluble in water (the solution is usually tan to brown), but practically insoluble in alcohol, in chloroform, and in ether. Major active principles (1) cellulose, (2) /3-glucanase, (3) /3-D-glucosidase, (4) hemicellulase, and (5) pentosanase. Typical applications used in the preparation of fruit juices, wine, vegetable oils, beer, and baked goods. 

The Cl enzymes, hydrocellulases, and Avicelases may now be correctly and more specifically termed cellobiohydrolases because of their action on cellooligosaccharides and Walseth cellulose. The Cx enzymes may now be classified as endo- or xo-glucanases, or /5-glucosidases. We therefore suggest abandoning the Ci-Cx terminology in favor of a more precise one based on the hydrolytic activities of the component enzymes. 

The mechanism of action of glucanases is strongly influenced by the shape and structure of the active site. exo-Enzymes normally have a pocket or a tunnel-shaped active site, which forces the enzyme to attack a chain end of the polysaccharide substrate. " The active site of endo-enzymes has the shape of a cleft. e polymeric backbone (provided that the active site can accommodate the sequence of tire polysaccharide). Furthermore, some glucanases also contain a binding domain, which enables reversible sorption onto (and, thus, hydrolysis of) nondissolved polysaccharides, such as granular starch and fibrous cellulose. 

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