Globulins, serum determination

From the analysis, it was found that proteins were not denatured during the pasteurisation temperature (72°C) for 15 seconds, while all the y-globulin, serum albumin, p-1actoglobulin and d-lacto-albumin was denatured upon heating at 85°C for 30 minutes. This technique was found to be useful for determining the individual protein composition of milk products and assessing the extent of denaturation of individual whey proteins. 

K. Murayama, K. Yamada, R. Tsenkova, Y. Wang, Y. Ozaki. Near-infrared spectra of serum albumin and y-globulin and determination of their concentrations in phosphate buffer solutions by partial least squares regression. Vib Spectrosc 18 33,1998. 

Copper appears as the a2-globulin ceruloplasmin in the human body (Sarkar 1994). Deficiency of this protein in serum is characteristic of both Menkes and Wilson s diseases. Wilson s disease is an abnormal storage of Cu(II) in body tissues. Cu(II) in biological material can be determined by spectrophotometry or by FAAS, ceruloplasmin in serum by a spectrophotometric method. 

Yoshika, S., Asu, Y., Kojima, Sh. Determination of molecular mobility of lyophilized bovine serum albumin and gamma-globulin by solid-state H1 NMR and relation to aggregation-susceptibility. Pharm. Res. 13 (6), p. 926-930, 1996... 

Bovine gamma globulin (BGG), total protein determination BCA assay, 84 (fig.) biuret assay, 88 (fig.), 98 Coomassie plus assay, 90 Bovine serum albumin (BSA), total protein determination BCA assay, 84 (fig.) biuret assay, 88 (fig.), 98 Coomassie plus assay, 90 protein standards, 78... 

In determining cortisol in serum, most of the cortisol is bound to corticosteroid-binding globulin and other serum proteins. How then is cortisol released for detection [1006] (2 marks)... 

The antibodies are similar in structure to other globulin proteins which are present in serum of vertebrates [9], The antibody molecule consists of two light polypeptide chains and two heavy polypeptide chains [10]. The amino acids are present in all the chains but the number of residues and the sequence will vary in different antibody multiforms [9], Light chains contain approximately 220 amino add residues and heavy chains about 450 residues. The complete sequence of the chains of human IgG myeloma protein has been determined by Edelman [11], The chains are held together in the unique conformational structure of the antibody molecule by a few covalent disulfide bonds between the chains and many electrostatic bonds between the amino groups of one chain and the hydroxyl groups of another chain. The covalent bonds are represented in Formula 1, 2 and 3 [peptide, disulfide and... 

The clinical biochemistry laboratory often determines total serum protein content and the albumin/globulin (A/G) ratio. A normal ambulatory adult will... 

Albumin is synthesized in the liver cell. The rate of synthesis is 12—17 g/day (150-250 mg/kg BW), which can be enhanced 3—4 times due to a loss of albumin. The albumin pool of the organism is approx. 500 g. Half-life is 18—21 days. Albumin is determined as a part of serum electrophoresis either quantitatively (g/l or g/dl, respectively) or as a relative percentage (rel. %). Concentrations of globulins are also quantified in this way. 

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